Etude de l'organisation et de la dynamique du nucléoïde de Deinococcus radiodurans par microscopie de fluorescence avancée

Abstract : During this PhD work, we have studied on D. radiodurans, a coccus, known for its intriguing outstanding resistance to different stress factors. Studies on D. radiodurans have been mainly focusing on its tremendous radioresistance. 52 years after its discovery, its nucleoid organization/segregation as well as its cell morphology during its cell cycle still remain elusive. Most of our knowledge on the bacteria shape during division and on the nucleoid organization/segregation arises from the study of a small number of “model bacteria”, that are mainly rod-shaped or ovoid. In contrast, little is known on the nucleoid organization/segregation of cocci. Moreover, the small relative size of bacteria and of their nucleoids (<1µm3) has limited their studies by conventional microscopy.Thus, one of the aims of this PhD project is to contribute to a better understanding of the cell morphology and the nucleoid organization/segregation in cocci. For that matter, we explored the 4D organization and the dynamics of D. radiodurans nucleoids, as a function of the cell cycle progression and growth phase. In order to achieve the objective of this PhD, several strategies were undertaken: (i) timelapse 3D stacks by spinning confocal microscopy (ii) dynamics studies with FRAP analysis and SptPALM acquisitions, and (iii) cartographies of nucleoid associated proteins using super-resolution microscopy (PALM).
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Submitted on : Thursday, May 23, 2019 - 9:32:07 AM
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Kevin Floc'H. Etude de l'organisation et de la dynamique du nucléoïde de Deinococcus radiodurans par microscopie de fluorescence avancée. Biologie structurale [q-bio.BM]. Université Grenoble Alpes, 2019. Français. ⟨NNT : 2019GREAV007⟩. ⟨tel-02137438⟩

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