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Purification et caractérisation des métabolites secondaires extraits de plantes de la famille des Asparagaceae et Caprifoliaceae, et évaluation de leurs activités biologiques

Abstract : This thesis was carried out in the Laboratory of Pharmacognosy, in health department of the University of Burgundy. The principal theme of this Laboratory is the research of natural compounds from tropical biodiversity, mainly saponins. These molecules are known for their various pharmacological activities. The study of 3 species belonging to 2 different families: Chlorophytum blepharophyllum Schweinf. ex Baker, Ornithogalum dubium Houtt (Asparagaceae) and Weigela × « kosteriana variegata » (Caprifoliaceae), led to the isolation and characterization of 16 natural glycosides by column chromatography, medium pressure liquid chromatography, and vacuum liquid chromatography. The spectral analysis was achieved using mainly 2D NMR and mass spectrometry. Among them, 4 were phenolic glucosides, 8 were steroidic saponins with 6 new structures and 4 were triterpenic saponins with one new structure. The cytotoxic activities of 5 isolated steroidic saponins were evaluated on 2 strains cancer cells (A549 and HL 60). The results showed a low sensitivity of these two cell lines to these saponins. The toxic and teratogenic effects of 3 triterpenic saponins were also determined in by using an in vivo zebrafish assay. The results showed a lethal effect at low concentrations of these 3 saponins. Structure / activity relationships have been proposed.
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Nampoina Andriamisaina Andriamasinoro. Purification et caractérisation des métabolites secondaires extraits de plantes de la famille des Asparagaceae et Caprifoliaceae, et évaluation de leurs activités biologiques. Médecine humaine et pathologie. Université Bourgogne Franche-Comté, 2018. Français. ⟨NNT : 2018UBFCE014⟩. ⟨tel-02134104⟩

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