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, Bacterial growth was monitored by Optical Density (OD) measurements with a microplate spectrophotometer (xMark, Bacterial inocula at a defined concentration were exposed to different BW concentrations in a 96-well microplate with a flat bottom and cover (BrandTech TM BRANDplates TM pureGrade TM S 96-well Microplates, vol.6
, Preparation of Bacterial Inocula Vibrio strains were kept at ?80 ? C in 25% glycerol. Broth cultures were prepared with an autoclaved, cation-adjusted
) and the Water (BW) Production The growth of the 30 Vibrio strains exposed to Blue Water (BW) solutions was studied over a 24 h period. BW was prepared from a concentrated supernatant of H. ostrearia culture containing the extracellular marennine, ) by the addition of 1% NaCl ,
Canada) during the spring of 2017. H. ostrearia was cultured in 100 L circular and flat bottom photobioreactors with filtered sea water ,
USA) on a syringe-filtered BW solution (0.2 µm; Sarstedt) and 1 cm path-length quartz cuvettes ,
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, Annexes ANNEXE, vol.5
, , 2018.
, , 2018.
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