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, En réponse à un appel d'offre du Département Hospitalo-Universitaire (DHU) I2B visant à financer l'amorçage de projets collaboratifs en recherche

P. Ritvo, Guillaume Churlaud 5 major publications: on CD4/CD8 Tregs and

G. Churlaud, F. Pitoiset, R. Lorenzon, B. Bellier, M. Rosenzwajg et al., Human and mouse CD8 + CD25 + FOXP3 + regulatory T cells at steady state and during IL-2 therapy, Frontiers in Immunology, 2015.
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M. Rosenzwajg, G. Churlaud, A. Hartemann, and D. Klatzmann, Interleukin-2 in the pathogenesis and therapy of type 1 diabetes. Current Diabetes reports, vol.14, p.55, 2014.

G. Churlaud, V. Jimenez, J. Ruberte, A. Zin, M. Fourcade et al., Sustained stimulation and expansion of Tregs by IL2 control autoimmunity without impairing immune responses to infection, vaccination and cancer, Clinical Immunology, vol.151, issue.2, pp.114-140, 2014.

T. Chen, G. Darrasse-jèze, A. S. Bergot, T. Courau, G. Churlaud et al., Self-specific memory regulatory T cells protects embryos at implantation in mice, Journal of Immunology, vol.191, issue.5, pp.2273-81, 2013.
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. Project-title-titre, Etude du rôle des lymphocytes T CD8 régulateurs dans la physiopathologie des maladies auto-immunes et sous traitement interleukine-2 à faible dose

(. Title and . English, Study of the pathophysiological role of CD8 regulatory T cells in autoimmunity and under

, Most treatments of these diseases are aimed at decreasing immunity. Recently, the therapeutic research has been focused on the activation and expansion of regulatory cells, particularly with the use of interleukin-2 (IL-2) that has been effective for the expansion of CD4 Treg and improvement of clinical signs

, Rational: Other regulatory cell populations have now received attention, and notably CD8 Treg for which evidence that they are involved in AIDs is growing. However, CD8 Treg are less well characterized than CD4 Treg. Objectives: We sought to deeply characterize CD8 + CD25 + FOXP3 + Treg in healthy donors (HD) and under pathological conditions (Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) patients) at steady state and under low-dose IL-2 treatment

, The aim of TRANSREG is the induction of Treg by low-dose IL-2 in AIDs. 10 mL of blood from 4 untreated HD and from 4 RA and 4 SLE patients (recruited in TRANSREG) before and after IL-2 treatment will be collected. CD8 Treg with be fully phenotyped by flow cytometry, purified by sorting and transcriptomic analysis will be performed, Methodology: TRANSREG is a clinical trial performed in Pitié-Salpêtrière hospital

, Team 1: Immunology -Immunopathology -Immunotherapy (I 3 ) laboratory, biotherapies department (Pitié-Salpêtrière hospital)

, Expertise in translational research in immunology and systems biology approaches. Contacts for the project: Dr

G. , Churlaud (guillaume.churlaud@aphp.fr) and PG. Ritvo (paulgydeon.ritvo@gmail.com)

, Clinical Investigation Center in Biotherapy and Immunology, vol.2

, This team has an expertise in the management of patients suffering from systemic lupus erythematosus and in managing the TRANSREG clinical trial

, Rheumatology Department, vol.3

, Expertise in the management of patients suffering from rheumatoid arthritis

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B. Bisikirska, J. Colgan, J. Luban, J. A. Bluestone, and K. C. Herold, TCR stimulation with modified anti-CD3 mAb expands CD8+ T cell population and induces CD8+CD25+ Tregs, J. Clin. Invest, vol.115, pp.2904-2913, 2005.

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