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Refactoring metabolic pathways for synthon production from renewable carbon sources

Abstract : Metabolic engineering, defined as the rational engineering of organisms towards production goals, has greatly evolved since its conception over three decades ago. Once applied to overproduce cell endogenous metabolites, it is now a promising approach also for the biosynthesis of non-natural compounds through the expression of synthetic metabolic pathways. Improved over billions of years by evolution, enzymes are however less adapted to new catalytic functions as required by synthetic metabolism. The present work was aimed at the construction and optimization of artificial routes for the biosynthesis of two industrially relevant commodity chemicals (L-2,4-dihydroxybutyrate and 1,3-propanediol) through the application of concepts of enzyme rational design, directed evolution and microbial engineering.
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Submitted on : Wednesday, April 17, 2019 - 4:57:06 PM
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  • HAL Id : tel-02102849, version 1

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Claudio Jose Remedios Frazao. Refactoring metabolic pathways for synthon production from renewable carbon sources. Agricultural sciences. INSA de Toulouse, 2018. English. ⟨NNT : 2018ISAT0037⟩. ⟨tel-02102849⟩

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