Skip to Main content Skip to Navigation
Theses

Détoxification d'hydolysats hémicellulosiques par dia-nanofiltration, étude de leur fermentescibilité et du mode d'action des inhibiteurs.

Abstract : Lignocellulosic biomass is a promising alternative to oil for the production of energy and high added value molecules. However, its exploitation requires the use of pretreatment processes. The most commonly used processes generate by-products which present inhibitory action on microorganisms (furan derivatives, aliphatic acids, phenolic compounds).The main goal of this thesis project is to answer the following question : “Is it possible to use nanofiltration to remove inhibitory compounds from hemicellulosic hydrolysate, in order to ferment the latter with Bacillus subtilis 168 ?”Determination of minimal inhibitory concentrations of these inhibitory compounds allowed to determine, for each one, the quantity to remove from the hydrolysate.Dia-nanofiltration with DK membrane (GE, USA) allowed to efficiently remove (more than 90%) furane derivatives of aliphatic acids. However, removal of phenolic compounds was more complexe, hence, only 25% of syringaldehyde could be removed. Though most indentified inhibitory compounds were removed, Bacillus subtilis 168 was still not able to ferment the hydrolysate.Flow cytometry showed that férulic acid and vanillin presented an impact on Bacillus subtilis 168 plasma membrane fluidity of polarisation.
Document type :
Theses
Complete list of metadatas

Cited literature [266 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02098627
Contributor : Abes Star :  Contact
Submitted on : Friday, April 12, 2019 - 6:13:08 PM
Last modification on : Tuesday, September 29, 2020 - 6:30:48 AM

File

66766_FAYET_2018_archivage2.pd...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02098627, version 1

Collections

Citation

Axel Fayet. Détoxification d'hydolysats hémicellulosiques par dia-nanofiltration, étude de leur fermentescibilité et du mode d'action des inhibiteurs.. Autre [q-bio.OT]. Université Paris Saclay (COmUE), 2018. Français. ⟨NNT : 2018SACLA008⟩. ⟨tel-02098627⟩

Share

Metrics

Record views

272

Files downloads

133