Skip to Main content Skip to Navigation
Theses

Analyse de la variabilité de l’expression génique et du métabolisme glycolytique au cours du processus de différenciation érythrocytaire : de l’analyse à grande échelle aux questions mécanistiques

Abstract : The meaning of cell decision making consists in the capacity of every living cell to integrate environmental information and to transform it in a coherent biological response. Nowadays it is increasingly demonstrated that cell populations present a significant quantitative and qualitative heterogeneity that could be involved in living organisms functions. Thus, the first part of my thesis consisted in studying gene expression variability at the single-cell level during the differentiation process of primary avian erythroid progenitor cells. The expression of 92 genes was analyzed using RT-qPCR in cells isolated at different differentiation time-points. The main results of this study showed that gene expression variability, as measured by Shannon entropy, reached a maximal level, simultaneously to a drop in the number of correlated genes, at 8-24h of differentiation. This increase of the gene expression variability preceded the irreversible commitment of cells into differentiation, identified between 24h and 48h. This analysis also highlighted the potential importance ofLDHA(Lactate dehydrogenase A) encoding a glycolytic enzyme, in erythroid progenitors self-renewal and at the critical differentiation time-point 8-24h. Therefore the second part of my thesis consisted in analyzing the role of LDHA in erythroid progenitors self-renewal and the variations of glucose metabolism during the differentiation process. Our first results suggested that erythroid differentiation might be accompanied with a metabolic change, corresponding to a switch from anaerobic glycolysisdepending upon LDHA, toward aerobic energy production, relying upon oxidative phosphorylation
Document type :
Theses
Complete list of metadatas

Cited literature [332 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02092005
Contributor : Abes Star :  Contact
Submitted on : Monday, April 8, 2019 - 1:16:09 PM
Last modification on : Friday, August 21, 2020 - 3:39:34 AM

File

TH2018RICHARDANGELIQUE.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02092005, version 2

Collections

Citation

Angélique Richard. Analyse de la variabilité de l’expression génique et du métabolisme glycolytique au cours du processus de différenciation érythrocytaire : de l’analyse à grande échelle aux questions mécanistiques. Biologie cellulaire. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSE1058⟩. ⟨tel-02092005v2⟩

Share

Metrics

Record views

186

Files downloads

188