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Stress and translation : implication of 16S rRNA methylations in Escherichia coli and characterization of a toxin-antitoxin system of Sinorhizobium meliloti

Abstract : Bacteria are able to live in a large variety of environments and they face constantly changing conditions. Therefore they have to adapt quickly to their metabolism using different regulations at the transcriptional and translational levels. Those types of regulation are extensively studied and well characterized. However, the implications of the ribosome in modulation of translation during stress response remains poorly understood. In this context of ribosomal regulation, the heterogeneity of the machinery could play a relevant role. Indeed, the ribosome is not an invariable particle and its components (rRNAs, r-proteins) and their modifications may vary. Modifications of ribosomal RNAs are clustered in the functional sites of the ribosome and are particularly conserved, underlying their potential importance. However their physiological role is still unclear. We focused on methylations of the 16S rRNA and investigated their role in translation under favourable and stressful conditions. We successfully demonstrated that lack of some methylations increases translation under stressful and non stressful conditions. So, lack of methylation may give an advantage to ribosomes during stress response. Another way to act on translation under stressful conditions resides in targeting mRNAs. This is particularly the case for endoribonuclease toxins that are specifically produced during detrimental conditions. Thus, we characterized S. meliloti toxin-antitoxin system HicAB. We plan to use it in order to study the response to HicA toxin of mutants lacking some ribosomal modifications.
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Manon Thomet. Stress and translation : implication of 16S rRNA methylations in Escherichia coli and characterization of a toxin-antitoxin system of Sinorhizobium meliloti. Human health and pathology. Université Rennes 1, 2018. English. ⟨NNT : 2018REN1B045⟩. ⟨tel-02090567⟩

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