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Cultures antifongiques applicables comme ferments de bioprotection dans les produits laitiers : sélection, évaluation à l'échelle pilote et identification de composés supports de l'activité

Abstract : Fungal contamination of dairy products is responsible for economic losses and food waste. In a context of “preservative-free” product demand, bioprotective cultures and their metabolites represe,t an alternative of interest of chemical preservatives to control these spoilers.The objective of this study was i) to select microorganisms exhibiting an antifungal activity, in order to elaborate bioprotectivecultures applicable in dairy products, and ii) to study the compounds potentially supporting the observed activity. Firstly, the antifungal activity of 32 strains of lactic acid and propionic bacteria screened in cheese model and yogurt. Strain combinaison study and safety assessment led to the selection of 2 binary lactobacilli combinations (A1 and A3). Their efficiency and applicability were then evaluated in pilot-scale productions of sour cream and cheese.Challenge and shelf life tests showed that depending on the dairy product, A1 and A3 have a similar or higher antifungal activity than the commercial bioprotective cultures. In addition, depending of inoculum, A1 and A3 did not impact the technological and organoleptic characteristics. Chromatographic methods and statistical analyses allowed identifying cocktails of 2 to 17 compounds, according to the considered dairy product and culture that probably support the antifungal activity.The obtained results contribute to a better understanding of the antifungal activity action mechanisms and should lead to the development of antifungal cultures to replace preservatives in dairy products.
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  • HAL Id : tel-02080859, version 1
  • PRODINRA : 463822

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Marcia Leyva Salas. Cultures antifongiques applicables comme ferments de bioprotection dans les produits laitiers : sélection, évaluation à l'échelle pilote et identification de composés supports de l'activité. Microbiologie et Parasitologie. Université de Bretagne occidentale - Brest, 2018. Français. ⟨NNT : 2018BRES0058⟩. ⟨tel-02080859⟩

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