Skip to Main content Skip to Navigation
Theses

Évolution de modèles tridimensionnels de peau reconstruite pour approfondir la connaissance des mécanismes du vieillissement cutané et validation de l’efficacité « anti-âge » du sélénium

Abstract : Skin and its aging is a public health issue. In vitro skin models available for the study aging remain perfectible. In this context, our objectives were simultaneously to use skin equivalent (SE) developed in our laboratory i) to better understand mechanisms of skin aging, ii) to demonstrate the effectiveness of selenium as “anti-aging” and finally iii) to improve SE using the porous or scaffold free model with fibroblasts from the same donor at different ages. Thus, the model of SE mimicking senescence showed an overexpression of microRNA miR30-a by RT qPCR in old SE with an alteration of the barrier function measured by the transepidermal water loss and a deficiency of epidermal terminal differentiation (decreased expression of loricrin and involucrin). With the same SE model, our results demonstrate that selenium supplementation delays the senescence of keratinocytes stem cells. This effectiveness does not involve antioxidant effect as expected but the activation of their adhesion to the basement membrane, which participates in preserving stemness and epidermal renewal. Finally, we had the opportunity to prepare SE with fibroblasts from a single donor at 36 and 72 years old. The histological results show that age induces an increase in the expression of elastin and fibrillin as well as their co-expression. The increase of LTBP1 and aSMA suggests that this unexpected increase is due to deregulation of the TGF-ß pathway and fibroblasts differentiation into myofibroblasts. In conclusion, the use of different models of SE helps us to explore some mechanisms leading to skin aging and to demonstrate the efficacy of selenium as “anti-aging”
Document type :
Theses
Complete list of metadatas

Cited literature [332 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02076186
Contributor : Abes Star :  Contact
Submitted on : Friday, March 22, 2019 - 1:02:06 AM
Last modification on : Friday, October 23, 2020 - 5:03:44 PM
Long-term archiving on: : Sunday, June 23, 2019 - 12:37:27 PM

File

TH2018JOBEILILARA.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02076186, version 1

Citation

Lara Jobeili. Évolution de modèles tridimensionnels de peau reconstruite pour approfondir la connaissance des mécanismes du vieillissement cutané et validation de l’efficacité « anti-âge » du sélénium. Biotechnologie. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSE1044⟩. ⟨tel-02076186⟩

Share

Metrics

Record views

332

Files downloads

2600