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Use of a synthetic substitute to animal products for rabbit and ovine embryo cryopreservation

Abstract : Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. The substitution of animal products of embryo cryopreservation media by synthetical products may improve procedure standardization (by avoiding variability in media composition) and avoid sanitary concerns inherent to animal-derived products.We aimed to evaluate the effect of replacing BSA in rabbit and ovine embryo slow freezing and vitrification media with a synthetic animal products free medium composed of synthetic hyaluronic acid: STEM ALPHA.Cryo3 (« CRYO3 »).During the first part, we evaluated the substitution candidates through a thermodynamic approach, using differential scanning calorimetry. In paralel, we adapted a mitochondrial activity evaluation protocol (JC-1) to rabbit embryo, which allowed us to complement morphological in vitro evaluation, and evaluated ewe superovulation protocols.During the second part, we used a biological approach to evaluate the replacement of BSA with synthetical products (containing CRYO3) in rabbit and ovine embryo slow freezing and vitrification media, using in vitro (slow freezing and vitrification) and in vivo (vitrification) evaluation methods.Our results seem to demonstrate that the chemically defined substitute CRYO3 can successfully replace BSA during rabbit embryo and ovine embryo cryopreservation (slow-freezing and vitrification)
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Magda Guedes Teixeira. Use of a synthetic substitute to animal products for rabbit and ovine embryo cryopreservation. Biotechnology. Université de Lyon, 2018. English. ⟨NNT : 2018LYSE1354⟩. ⟨tel-02073597⟩

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