Y. Ito, Origin and evolution of the coil planet centrifuge: a personal reflection of my 40 years of CCC research and development, Sep. Purifi. Rev, vol.34, pp.131-154, 2005.

L. C. Craig and O. Post, Apparatus for countercurrent distribution, Anal. Chem, vol.21, pp.500-504, 1949.

W. D. Conway, Counter-current chromatography: simple process and confusing terminology, J. Chromatogr. A, vol.1218, pp.6015-6023, 2011.

S. M. Guido, F. Pauli, and J. B. Friesen, Countercurrent separation of natural products, J. Nat. Prod, vol.71, pp.1489-1508, 2008.

Y. Ito and J. Menet, Coil planet centrifuges for High-Speed countercurrent chromatography, Countercurrent Chromatography, Marcel Dekker, pp.87-119, 1999.

A. Berthod, T. Maryutina, B. Spivakov, O. Shpigun, and I. A. Sutherland, Countercurrent chromatography in analytical chemistry: (IUPAC technical report), p.355, 2009.
URL : https://hal.archives-ouvertes.fr/hal-01560747

A. Berthod and K. Talabardon, Centrifugal Partition Chromatography: operating parameters and partition coefficient determination, Countercurrent Chromatography, Marcel Dekker, pp.121-148, 1999.

W. D. Conway, Countercurrent Chromatography: Apparatus, Theory and Applications, 1990.

A. Berthod, Countercurrent Chromatography the support-free liquid stationary phase, Comprehensive Analytical Chemistry, 2002.

K. Skalicka-wozniak and I. Garrard, A comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatograhy, Nat. Prod. Rep, pp.1556-1561, 2015.

A. Berthod, M. J. Ruiz-Ángel, and S. Carda-broch, Countercurrent chromatography: people and applications, J. Chromatogr. A, vol.1216, pp.4206-4217, 2009.
DOI : 10.1016/j.chroma.2008.10.071

R. Hu and Y. Pan, Recent trends in counter-current chromatography, TrAC Trends Anal. Chem, vol.40, pp.15-27, 2012.

J. B. Brent-friesen, S. Chen, and G. F. Pauli, Countercurrent separation of natural products: an update, J. Nat. Prod, vol.78, pp.1765-1796, 2015.

L. Yin, Y. Li, B. Lu, Y. Jia, and J. Peng, Trends in counter-current chromatography: applications to natural products purification, Purif. Rev, vol.39, pp.33-62, 2010.

T. Michel, E. Destandau, and C. Elfakir, New advances in countercurrent chromatography and centrifugal partition chromatography: focus on coupling strategy, Anal. Bioanal. Chem, vol.406, pp.957-969, 2014.

X. Xie, W. Sun, J. Huang, N. Polachi, L. Tong et al., Preparative high performance liquid chromatography-based multidimensional chromatography and its application in traditional chinese medicine, Chin. J. Anal. Chem, vol.44, pp.1140-1147, 2016.

T. Kapp and W. Vetter, Offline coupling of high-speed counter-current chromatography and gas chromatography/mass spectrometry generates a two-dimensional plot of toxaphene components, J. Chromatogr. A, vol.1216, pp.8391-8397, 2009.

T. Inui, Y. Wang, S. M. Pro, S. G. Franzblau, and G. F. Pauli, Unbiased evaluation of bioactive secondary metabolites in complex matrices, Fitoterapia, vol.83, pp.1218-1225, 2012.

D. R. Stoll, X. Li, X. Wang, P. W. Carr, S. E. Porter et al., Fast, comprehensive two-dimensional liquid chromatography, J. Chromatogr. A, vol.1168, pp.3-43, 2007.

P. Dugo, F. Cacciola, T. Kumm, G. Dugo, and L. Mondello, Comprehensive multidimensional liquid chromatography: theory and applications, J. Chromatogr. A, vol.1184, pp.353-368, 2008.

G. Guiochon, N. Marchetti, K. Mriziq, and R. A. Shalliker, Implementations of two-dimensional liquid chromatography, J. Chromatogr. A, vol.1189, pp.109-168, 2008.

I. Franç-ois, K. Sandra, and P. Sandra, Comprehensive liquid chromatography: fundamental aspects and practical considerations-a review, Anal. Chim. Acta, vol.641, pp.14-31, 2009.

P. J. Marriott, Z. Wu, and P. Schoenmakers, Nomenclature and conventions in comprehensive multidimensional chromatography -an update, LCGC Eur, p.25, 2012.

M. Gilar, P. Olivova, A. E. Daly, and J. C. Gebler, Orthogonality of separation in two-Dimensional liquid chromatography, Anal. Chem, vol.77, pp.6426-6434, 2005.

M. Gilar, J. Fridrich, M. R. Schure, and A. Jaworski, Comparison of orthogonality estimation methods for the two-Dimensional separations of peptides, Anal. Chem, vol.84, pp.8722-8732, 2012.

P. J. Slonecker, X. Li, T. H. Ridgway, and J. G. Dorsey, Informational orthogonality of two-Dimensional chromatographic separations, Anal. Chem, vol.68, pp.682-689, 1996.

L. I. Zaiyou, D. G. Patterson, and M. L. Lee, Geometric approach to factor analysis for the estimation of orthogonality and practical peak capacity in comprehensive two-dimensional separations (English), Anal. Chem, pp.3840-3845, 1995.

M. J. Dobner, S. Schwaiger, I. H. Jenewein, and H. Stuppner, Antibacterial activity of leontopodium alpinum (Edelweiss), J. Ethnopharmacol, vol.89, pp.301-303, 2003.

S. Schwaiger, M. Adams, C. Seger, E. P. Ellmerer, R. Bauer et al., New constituents of Leontopodium alpinum and their in vitro leukotriene biosynthesis inhibitory activity, vol.70, pp.978-985, 2004.

M. J. Dobner, S. Sosa, S. Schwaiger, G. Altinier, R. Della-loggia et al., Anti-inflammatory activity of Leontopodium alpinum and its constituents, vol.70, pp.502-508, 2004.

M. Dobner, J. E. Ellmerer, P. S. Schwaiger, O. Batsugkh, S. Narantuya et al., New lignan benzofuran, and sesquiterpene derivatives from the roots of Leontopodium alpinum and L. leontopodioides, vol.86, p.733, 2003.

S. Schwaiger, S. Hehenberger, E. P. Ellmerer, and H. Stuppner, A new bisabolane derivative of Leontopodium andersonii, vol.5, pp.667-668, 2010.

S. Schwaiger, R. Cervellati, C. Seger, E. P. Ellmerer, N. About et al., Leontopodic acid-a novel highly substituted glucaric acid derivative from Edelweiss (Leontopodium alpinum Cass.) and its antioxidative and DNA protecting properties, Tetrahedron, vol.61, pp.4621-4630, 2005.

L. Daniela, P. Alla, R. Maurelli, D. Elena, P. Giovanna et al., Anti-inflammatory effects of concentrated ethanol extracts of edelweiss (Leontopodium alpinum Cass.) callus cultures towards human keratinocytes and endothelial cells, Mediators Inflamm, p.498373, 2012.

S. Costa, S. Schwaiger, R. Cervellati, H. Stuppner, E. Speroni et al., In vitro evaluation of the chemoprotective action mechanisms of leontopodic acid against aflatoxin B1 and deoxynivalenol-induced cell damage, J. Appl. Toxicol.: JAT, vol.29, pp.7-14, 2009.

S. Schwaiger, C. Seger, B. Wiesbauer, P. Schneider, E. P. Ellmerer et al., Development of an HPLC-PAD-MS assay for the identification and quantification of major phenolic edelweiss (Leontopodium alpium Cass.) constituents, Phytochem. Anal, vol.17, pp.291-298, 2006.

M. Ganzera, V. Greifeneder, S. Schwaiger, and H. Stuppner, Chemical profiling of Edelweiss (Leontopodium alpinum Cass.) extracts by micellar electrokinetic capillary chromatography, Fitoterapia, vol.83, pp.1680-1686, 2012.

C. Yang, Q. Tang, J. Liu, Z. Zhang, and W. Liu, Preparative isolation and purification of phenolic acids from Smilax china by high-speed counter-current chromatography, Sep. Purif. Technol, vol.61, pp.474-478, 2008.

H. E. Dajun, G. U. Dongyu, H. Yun, A. Ayupbek, Y. Yi et al., Separation and purification of phenolic acids and myricetin from black currant by high-speed countercurrent chromatography, J. Liq. Chromatogr. Relat. Technol, vol.32, pp.3077-3088, 2009.

W. U. Hao, F. Ruihong, G. Shuguang, Y. U. Wenbo, M. A. Wei et al., Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool, J. Sep. Sci, vol.35, pp.1160-1166, 2012.

F. Li, Z. Gou, X. Ma, T. Yan, T. Ge et al., Preparative isolation and purification of two phenylpropanoids from Daphne giraldii Nitsche by HSCCC, Chromatographia, vol.71, pp.481-485, 2010.

A. Berthod, T. Maryutina, B. Spivakov, O. Shpigun, and I. A. Sutherland, Countercurrent chromatography in analytical chemistry: (IUPAC technical report), p.355, 2009.
URL : https://hal.archives-ouvertes.fr/hal-01560747

D. Wang, L. Chen, J. Liu, X. Wang, Y. Wu et al., On-line two-dimensional countercurrent chromatography × high performance liquid chromatography system with a novel fragmentary dilution and turbulent mixing interface for preparation of coumarins from Cnidium monnieri, J. Chromatogr. A, vol.1406, pp.215-223, 2015.

Y. Qiu, X. Yan, M. Fang, L. Chen, Z. Wu et al., Two-dimensional countercurrent chromatography × high performance liquid chromatography for preparative isolation of toad venom, J. Chromatogr. A, vol.1331, pp.80-89, 2014.

T. Michel, E. Destandau, L. Fougère, and C. Elfakir, New 'hyphenated' CPC-HPLC-DAD-MS strategy for simultaneous isolation, analysis and identification of phytochemicals: application to xanthones from Garcinia mangostana, Anal. Bioanal. Chem, vol.404, pp.2963-2972, 2012.

T. Michel, E. Destandau, and C. Elfakir, On-line hyphenation of centrifugal partition chromatography and high pressure liquid chromatography for the fractionation of flavonoids from Hippophaë rhamnoides L. berries, J. Chromatogr. A, vol.1218, pp.6173-6178, 2011.

J. Liu, X. Wang, L. Zhang, M. Fang, Y. Wu et al., Two-dimensional countercurrent chromatography × high performance liquid chromatography with heart-cutting and stop-and-go techniques for preparative isolation of coumarin derivatives from Peucedanum praeruptorum Dunn, J. Chromatogr. A, vol.1374, pp.156-163, 2014.

D. Chen, J. Fan, P. Wang, L. Zhu, Y. Jin et al., Isolation, identification and antioxidative capacity of water-soluble phenylpropanoid compounds from Rhodiola crenulata, Food Chem, vol.134, pp.2126-2133, 2012.

Y. Ito, Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography, J. Chromatogr. A, vol.1065, pp.145-168, 2005.

R. Grassi-zampieron, C. A. Franç-a, M. C. Vieira, A. Oliveros-bastidas, and J. M. Siqueira, Comparative profiles of Achyrocline alata (Kunth) DC. and A. satureioides (Lam.) DC., Asteraceae, applying HPLC-DAD-MS, Revista Brasileira de Farmacognosia, vol.20, pp.575-579, 2010.

E. J. Lee, J. S. Kim, H. P. Kim, J. Lee, and S. S. Kang,

P. Dugo, F. Cacciola, T. Kumm, G. Dugo, and L. Mondello, J. Chromatogr. A, vol.1184, pp.353-368, 2008.

M. Iguiniz and S. Heinisch, J. Pharm. Biomed. Anal, vol.145, pp.482-503, 2017.

Y. Qiu, F. Chen, L. Zhang, X. Yan, L. Chen et al., Anal. Chim. Acta, vol.820, pp.176-186, 2014.

X. Wang, J. Li, Y. Jian, Z. Wu, M. Fang et al., J. Chromatogr. A, vol.1387, pp.60-68, 2015.

J. Li, H. Fang, X. Yan, F. Chang, Z. Wu et al., J. Chromatogr. A, pp.169-175, 1456.

Y. Qiu, X. Yan, M. Fang, L. Chen, Z. Wu et al., J. Chromatogr. A, pp.80-89, 1331.

X. Xie, W. Sun, J. Huang, N. Polachi, L. Tong et al., Chin. J. Anal. Chem, vol.44, pp.1140-1147, 2016.

X. Yan, L. Wang, Z. Wu, Y. Wu, X. Liu et al., J. Chromatogr. B, pp.1033-1034, 2016.

W. Su, Q. Liu, Q. Yang, J. Yu, and X. Chen, J. Sep. Sci, vol.36, pp.3338-3344, 2013.

Q. Liu, S. Shi, L. Liu, H. Yang, W. Su et al., J. Chromatogr. A, pp.183-193, 1304.

S. Jiang, Q. Liu, Y. Xie, H. Zeng, L. Zhang et al., , vol.186, pp.153-159, 2015.

L. Marlot, K. Faure, and J. Chromatogr, , vol.1494, pp.1-17, 2017.

C. Deamicis, N. A. Edwards, M. B. Giles, G. H. Harris, P. Hewitson et al., J. Chromatogr. A, pp.6122-6127, 1218.

S. Li, W. Wang, H. Tang, K. Chen, J. Yang et al., J. Chromatogr. A, vol.1344, pp.51-58, 2014.

M. J. Dobner, S. Schwaiger, I. H. Jenewein, and H. Stuppner, J. Ethnopharmacol, vol.89, pp.301-303, 2003.

M. J. Dobner, S. Sosa, S. Schwaiger, G. Altinier, R. Della-loggia et al., Planta Med, vol.70, pp.502-508, 2004.

S. Schwaiger, R. Cervellati, C. Seger, E. P. Ellmerer, N. About et al., Tetrahedron, vol.61, pp.4621-4630, 2005.

L. Marlot, M. Batteau, M. Escofet, S. Nuccio, V. Coquoin et al., , vol.1504, pp.55-63, 2017.

S. R. Groskreutz, M. M. Swenson, L. B. Secor, and D. R. Stoll, J. Chromatogr. A, vol.1228, pp.31-40, 2012.

S. Golshan-shirazi and G. Guiochon, J. Chromatogr. A, vol.536, pp.57-73, 1991.

J. C. Giddings, Anal. Chem, vol.39, pp.1027-1028, 1967.

A. D'attoma, C. Grivel, and S. Heinisch, J. Chromatogr. A, vol.1262, pp.148-159, 2012.

L. R. Snyder, J. W. Dolan, and J. R. Gant, J. Chromatogr. A, vol.165, pp.3-30, 1979.

I. Franç-ois, K. Sandra, and P. Sandra, Anal. Chim. Acta, vol.641, pp.14-31, 2009.

J. M. Davis and J. C. Giddings, Anal. Chem, vol.55, pp.418-424, 1983.

G. Semard, V. Peulon-agasse, A. Bruchet, J. Bouillon, and P. Cardinä-el, J. Chromatogr. A, pp.5449-5454, 2010.

D. R. Stoll, X. Li, X. Wang, P. W. Carr, S. E. Porter et al., J. Chromatogr. A, vol.1168, pp.3-43, 2007.

R. E. Murphy, M. R. Schure, and J. P. Foley, Anal. Chem, vol.70, pp.1585-1594, 1998.

F. Bedani, P. J. Schoenmakers, H. Janssen, and J. , , vol.35, pp.1697-1711, 2012.

J. M. Davis, D. R. Stoll, and P. W. Carr, Anal. Chem, vol.80, pp.461-473, 2008.

J. V. Seeley and J. Chromatogr, , vol.962, pp.21-27, 2002.

N. E. Hoffman, S. Pan, and A. M. Rustum, J. Chromatogr. A, vol.465, pp.189-200, 1989.

K. J. Mayfield, R. A. Shalliker, H. J. Catchpoole, A. P. Sweeney, V. Wong et al., J. Chromatogr. A, vol.1080, pp.124-131, 2005.

S. Keunchkarian, M. Reta, L. Romero, and C. Castells, J. Chromatogr. A, vol.1119, pp.20-28, 2006.

E. Sommella, O. H. Ismail, F. Pagano, G. Pepe, C. Ostacolo et al., , vol.40, pp.2188-2197, 2017.

A. F. Gargano, M. Duffin, P. Navarro, and P. J. Schoenmakers, Anal. Chem, vol.88, pp.1785-1793, 2016.

P. Jandera, P. Cesla, T. H-´-ajek, G. Vohralík, K. Vy?uchalovávy?vy?uchalovvy?uchalov´vy?uchalová et al., J. Chromatogr. A, vol.1189, pp.207-220, 2008.

G. Vivóviv´vivó-truyols, S. Van-der-wal, and P. J. Schoenmakers, Anal. Chem, vol.82, pp.8525-8536, 2010.

M. R. Schure, Anal. Chem, vol.71, pp.1645-1657, 1999.

M. Sarrut, G. Crétiercr´crétier, and S. Heinisch, TrAC Trends Anal. Chem, vol.63, pp.104-112, 2014.

A. Seidel-morgenstern, M. Schulte, and A. Epping, Preparative Chromatography, pp.7-46, 2012.

?. Experimental-section-reagents and M. , Ethanol and acetonitrile were from SigmaAldrich (Saint-Quentin-Fallavier, France). C. genistoides plant material (leaves and fine stems) were harvested from a commercial plantation (Gouritzmond, Western Cape, South Africa). The plant material was dried, and a hot water extract was prepared as described by Beelders et al. 15 Instrumentation. The HPLC system was an Alliance 2690 system from Waters, All solvents were of analytical grade. 1-Butanol, formic acid, sucrose, and ammonium sulfate were purchased from Acros Organics

, A and B); as follows: LC1 method: XSelect CSH PhenylHexyl column, 150 mm × 4.6 mm i.d., 5 ?m, from Waters; flow rate 2 mL/min, gradient time 17.6 min, total time 25 min. LC2 method: Gemini-NX C18 column

A. Corgier, M. Sarrut, G. Cre?tiercre?tier, S. Heinisch, and . Chromatographia, , vol.79, pp.255-260, 2016.

A. Wehrli, U. Hermann, and J. F. Huber, Journal of Chromatography A, vol.125, pp.59-70, 1976.

L. Marlot and K. Faure, Journal of Chromatography A, pp.1-17, 1494.

Q. Fu, Z. Guo, X. Zhang, Y. Liu, and X. Liang, J. Sep. Sci, vol.35, pp.1821-1827, 2012.

K. Li, W. Zhu, Q. Fu, Y. Ke, Y. Jin et al., Analyst, vol.138, 2013.

Y. Qiu, F. Chen, L. Zhang, X. Yan, L. Chen et al., Z. Anal. Chim. Acta, vol.820, pp.176-186, 2014.

X. Wang, J. Li, Y. Jian, Z. Wu, M. Fang et al., Journal of Chromatography A, pp.60-68, 1387.

J. Li, H. Fang, X. Yan, F. Chang, Z. Wu et al., Journal of Chromatography A, pp.169-175, 1456.

X. Yan, L. Wang, Z. Wu, Y. Wu, X. Liu et al., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci, pp.1033-1034, 2016.

F. Yunpeng, F. Yanhui, F. Qing, C. Jianfeng, X. Huaxia et al., , 2016.

N. A. Walters, A. De-villiers, E. Joubert, and D. De-beer, Journal of Chromatography A, pp.102-114, 1490.

A. Le-masle, S. Santin, L. Marlot, L. Chahen, and N. Charon, Anal. Chim. Acta, pp.1029-116, 2018.

D. R. Stoll, X. Li, X. Wang, P. W. Carr, S. E. Porter et al., Journal of Chromatography A, pp.3-43, 1168.

E. Joubert, M. E. Joubert, C. Bester, D. De-beer, and . De-lange, J. H. S. Afr. J. Bot, vol.77, pp.887-907, 2011.

T. Beelders, D. De-beer, M. Stander, and E. Joubert, Molecules, vol.19, 2014.

A. Kokotkiewicz, M. Luczkiewicz, J. Pawlowska, P. Luczkiewicz, P. Sowinski et al., Fitoterapia, vol.90, 2013.

G. Ntlhokwe, M. Muller, E. Joubert, A. G. Tredoux, and . De-villiers, A. Journal of Chromatography A, pp.137-150, 1536.

A. D'attoma, C. Grivel, and S. Heinisch, Journal of Chromatography A, pp.1262-148, 2012.

U. D. Neue, J. E. O'gara, and A. Me?ndezme?ndez, Journal of Chromatography A, pp.161-174, 1127.

E. Van-gyseghem, S. Van-hemelryck, M. Daszykowski, F. Questier, D. L. Massart et al., Journal of Chromatography A, vol.988, pp.77-93, 2003.

Z. Liu, D. G. Patterson, and M. L. Lee, Anal. Chem, vol.67, pp.3840-3845, 1995.

P. J. Slonecker, X. Li, T. H. Ridgway, and J. G. Dorsey, Anal. Chem, vol.68, pp.682-689, 1996.

M. Gilar, P. Olivova, A. E. Daly, and J. C. Gebler, Anal. Chem, vol.77, pp.6426-6434, 2005.

, Analytical Chemistry Article

, Anal. Chem, vol.90, pp.14279-14286, 2018.

G. Semard, V. Peulon-agasse, A. Bruchet, and J. Bouillon, P. Journal of Chromatography A, pp.5449-5454, 2010.

W. Nowik, S. He?ronhe?ron, M. Bonose, M. Nowik, and A. Tchapla, Anal. Chem, vol.85, pp.9449-9458, 2013.

W. Nowik, M. Bonose, S. He?ronhe?ron, M. Nowik, and A. Tchapla, Anal. Chem, vol.85, pp.9459-9468, 2013.

P. J. Marriott, Z. Wu, P. Schoenmakers, and . Europe, , vol.25, pp.266-275, 2012.

L. Marlot, M. Batteau, and K. Faure, Electrophoresis, vol.39, 2011.

F. Bedani, P. J. Schoenmakers, and H. Janssen, J. Sep. Sci, vol.35, pp.1697-1711, 2012.

P. Jandera, P. C-?-esla, T. Ha?jekha?jek, G. Vohralík, . Vyn? et al., J. Journal of Chromatography A, 1189.

A. Seidel-morgenstern, M. Schulte, and A. Epping, Preparative Chromatography, pp.7-46, 2012.

L. Marchal, O. Intes, A. Foucault, J. Legrand, J. Nuzillard et al., Journal of Chromatography A, pp.51-62, 1005.

, Analytical Chemistry Article

, Anal. Chem, vol.90, pp.14279-14286, 2018.