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Dispositif d’aiguille fibrée pour la spectroscopie de fluorescence endogène de lésions mammaires et pulmonaires ex vivo et in vivo ; vers le développement d'une méthode d’ histopathologie in situ

Abstract : The third Cancer Plan, launched in 2013, identifies early diagnosis as one of the major challenges for improving patient care. Despite the growth in medical imaging modalities and performance, challenges remain in diagnosis aid and optimizing the use of biopsy.Photonic imaging and especially spectrally resolved fluorescence has already been tested for the ex vivo characterization of breast and lung tumors, without contrast agent or sample processing. Our goal is to characterize the capabilities of an innovative medical device, developed in the laboratory, using a low-caliber fibered needle for the spectral analysis of the endogenous fluorescence of these lesions in situ. Our early work in preclinical and clinical studies showed significant differences in spectral signatures between benign and malignant tumors ex vivo and in vivo. Our results also highlighted the limits the device, in terms of specificity, for certain types of lesions.Another study was conducted on mammary tumors in order to identify the major tissue entities at the origin of the spectral signatures obtained with our fibered device. Spectral imaging in confocal and second harmonic microscopy (SHG), in multiphoton, has been implemented in order to establish a mapping of endogenous biomarkers of mammary tissues. We compare its results with the data obtained with the fibered needle device in order to position it not only as an aid to diagnosis but also as a promising method for in situ histopathology.
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https://tel.archives-ouvertes.fr/tel-02062546
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Submitted on : Saturday, March 9, 2019 - 1:01:14 AM
Last modification on : Wednesday, October 14, 2020 - 4:00:25 AM
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Alexis Toullec. Dispositif d’aiguille fibrée pour la spectroscopie de fluorescence endogène de lésions mammaires et pulmonaires ex vivo et in vivo ; vers le développement d'une méthode d’ histopathologie in situ. Autre. Université Paris-Saclay, 2018. Français. ⟨NNT : 2018SACLS154⟩. ⟨tel-02062546⟩

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