Conception et réalisation de microdispositifs électrochimiques, pour l'analyse de l'activité bioénergétique de mitochondries isolées, dans le cadre de la mise au point de traitements innovants des leucémies aiguës myéloïdes

Abstract : The role of mitochondria have been restricted to oxidative phosphorylation for a long time. Now it is clear that they are also the main sources of reactive oxygen species, implied in oxidative stress and cell-to-cell signaling. Thus, mitochondrial malfunction is potentially the cause of the appearance and the progression of diseases linked to ageing like cancers and neurodegenerative troubles. In the frame of acute myeloid leukemia, studies governed by Jean-Emmanuel Sarry of the Cancer Research Center of Toulouse, showed that it is possible to improve the efficacy of current chemotherapies by targeting mitochondria's function. In this context, the objective of the thesis presented here consist in the design and the manufacturing of electrochemical micro-sensors, dedicated to the analysis of the metabolic activity of isolated mitochondria. The manufacturing occurred in the clean room facilities of the Laboratory for Analysis and Architecture of Systems of Toulouse under the supervision of Jérôme Launay and Pierre Temple-Boyer, researchers specialized in the development of solutions aiming the detection of species diluted in solution. Finally, a complete system ensuring the coupling with microscopy and fluidics have been realized, validated, and patented. The results obtained allow us to consider the analysis at the scale of the single mitochondrion with a parallelized approach, thing that have never been made.
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Submitted on : Monday, August 13, 2018 - 10:38:03 AM
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Gabriel Lemercier. Conception et réalisation de microdispositifs électrochimiques, pour l'analyse de l'activité bioénergétique de mitochondries isolées, dans le cadre de la mise au point de traitements innovants des leucémies aiguës myéloïdes. Biotechnologie. Université Paul Sabatier - Toulouse III, 2018. Français. ⟨NNT : 2018TOU30054⟩. ⟨tel-02059371v2⟩

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