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Étude de la flore bactérienne et de sa résistance aux antibiotiques des produits de la pêche et de l'aquaculture

Abstract : Antimicrobial resistance is a threat to global public health. Human can be contaminated by antibiotic resistant bacteria through food. However, data on antimicrobial resistant bacteria in seafood are scarce. The aim of this thesis was to study seafood bacterial flora and its antimicrobial resistance. First, mesophilic flora was obtained from 9 matrixes and MALDI-TOF and housekeeping genes sequencing technics were used to identify isolates. Antimicrobial resistance of most frequently bacteria were tested. In total, 1882 isolates were obtained and 150 bacterial species and 57 genera were identified. Enterococcus, Staphylococcus, Exiguobacterium, Pseudomonas, Vibrio and Proteus were most frequently isolated and their antimicrobial resistance was studied. Antibiotic resistant bacteria accounted for 46% of isolates and multidrug resistant bacteria accounted for 3% of isolates. Antimicrobial resistant bacteria were mostly isolated from shrimps. On a side study, antimicrobial resistance of a V.parahaemolyticus strain collection isolated from seafood was characterized. Antimicrobial resistant strains accounted for 15% and multi-drug resistant bacteria accounted for 3%. A NDM-1-producing multidrug resistant strain, 16-B3PA-006 was identified from shrimps imported from South-East Asia.
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Submitted on : Friday, February 15, 2019 - 3:57:06 PM
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Arnaud Briet. Étude de la flore bactérienne et de sa résistance aux antibiotiques des produits de la pêche et de l'aquaculture. Sciences et techniques des pêches. Université du Littoral Côte d'Opale, 2018. Français. ⟨NNT : 2018DUNK0494⟩. ⟨tel-02021052⟩



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