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Développement de méthodes bidimensionnelles en ligne LCxLC-UV/MS et LCxSFC-UV pour l’analyse de composés pharmaceutiques

Abstract : Two-dimensional liquid chromatography (2D-LC) is a powerful technique considering its high separation power. After showing the advantage of 2D-LC in the pharmaceutical area and presenting the challenges related to quantitative analysis, special attention was paid to method development. With the aim of developing a generic analytical strategy for pharmaceuticals, the first step of our approach consisted in selecting a set of three 2D-systems with the help of a methodology previously developed. In a second step, the potential of these 2D-systems was evaluated for the purpose of quantitative analysis. An analytical strategy able to be applied to pharmaceutical analysis in an industrial context was proposed. Finally, the potential of RPLCxSFC was investigated in two different cases. Firstly, for comparing this on-line two dimensional technique to on-line RPLCxRPLC with respect of the separation power. Secondly, for chiral compounds by developing a selective RPLCxSFC method for simultaneous achiral-chiral analysis. The advantage of such method was highlighted by comparing to conventional approaches
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https://tel.archives-ouvertes.fr/tel-02020711
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Marion Iguiniz. Développement de méthodes bidimensionnelles en ligne LCxLC-UV/MS et LCxSFC-UV pour l’analyse de composés pharmaceutiques. Chimie analytique. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSE1200⟩. ⟨tel-02020711⟩

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