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Rôles coopératifs du peptidoglycane et des acides téichoïques dans le remodelage de la paroi et la division cellulaire de Streptococcus pneumoniae

Abstract : Gram-positive bacteria cell wall (CW) is composed by peptidoglycan (PG) and teichoic acids (TA). We studied both CW components and revealed new regulation mechanisms in the human pathogen Streptococcus pneumoniae. We showed that PG O-acetylation occurs in the early steps of PG biosynthesis, promotes the formation of mature PG and plays a role in cell division. We developed an innovative click chemistry-based approach to label TA in live cells, opening the way to explore mechanistic issues of pneumococcal TA biosynthesis. We revealed that TA synthesis occurs at the division site and is correlated with PG synthesis. Finally, we showed that both PG and TA polymers contribute to regulate the major autolysin LytA which binds TA and cleaves the PG: the O-acetylation of PG protects dividing cells from LytA-induced autolysis while TA finely regulates LytA surface localization. In conclusion, our work highlights the cooperative role of PG and TA in CW biosynthesis, cell division and regulation of surface components.
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Submitted on : Wednesday, February 6, 2019 - 1:01:55 AM
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Julie Bonnet. Rôles coopératifs du peptidoglycane et des acides téichoïques dans le remodelage de la paroi et la division cellulaire de Streptococcus pneumoniae. Sciences agricoles. Université Grenoble Alpes, 2017. Français. ⟨NNT : 2017GREAV034⟩. ⟨tel-02008878⟩

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