. .. Acknowledgements, 9 3.1 Genome-wide distribution of histone methylation

.. .. Erasers-of-histone-methylation,

. , 31 5. 2 Crosstalk between H3 methylation and H2A/H2B monoubiquitination

. , 37 6.3 Flowering time regulation via chromatin modifications

. .. Objectives-of-thesis,

C. I. ,

C. .. Ii, Crosstalk of different histone modifications in the regulation of gene transcription and flowering time control in Arabidopsis thaliana

. , 74 2.5.2 Expression profiles of flowering time genes, 5.1 Flowering time of sdg8-1 hub2

. , Transcriptome analysis of sdg8-1 hub2-2 double mutants, vol.87

C. .. Discussion,

. .. Arabidopsis, 91 3.4 Crosstalk between histone methylation and monoubiquitination during transcription, p.91

. , H3K36me2/3 and H2Bub1 in regulation of flowering time gene chromatin status

.. .. Supplementary,

C. .. Iii,

. , 101 2.1 Test HULK2 and SDG8 interaction by pull-down assay

. , 108 2.5.3 Flowering time analysis of sdg8-1 hua2-7 mutants, The HUA2/ HULK2 PWWP-domain exhibits in vitro histone peptide binding capacity ..... 103 2.4 Protein crystallography analysis of HUA2 PWWP-domain

C. .. Discussion, 111 3.1. Functional and structural analysis of the HUA2/HULK2 PWWP domain

G. Conclusions and . .. Perspective,

. Materials and . .. Methods,

. .. Methods, 2.1.11 SDS (sodium dodecyl suLfate)-polyacrylamide gel electrophoresis (PAGE)

.. .. Bacterial, 135 2.2.1 Preparation of competent cells for heat shock using CaCl2

. , In vitro binding assay (using peptide) and dot western

R. .. ,

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