Exploitation d'une biobanque de patients atteints de Trypanosomose Humaine Africaine à Trypanosoma brucei gambiense : recherche et validation de biomarqueurs

Abstract : Sleeping sickness, or Human African Trypanosomiasis (HAT), is a parasitic disease caused by a flagellar protozoan of the genus Trypanosoma and brucei species. Two subspecies of this parasite are pathogenic for humans: T. b. gambiense and T. b. rhodesiense; transmitted by Tsé-Tse flies present in sub-Saharan Africa. This disease classically evolves in two stages: the hemolymphatic stage which is define by the presence of the parasite in the blood and lymph and the nervous stage characterized by the presence of trypanosome in the central nervous system. Without treatment, this disease is lethal. Currently the available treatments for patients are stage-dependent. In order to control this pathology one day, research and improvement of tools for the diagnosis of the disease and the staging is fundamental. In this context, we have exploited a samples biobank composed of T. b. gambiense-infected patients and uninfected controles to: 1) evaluate the efficacy of existing stage biomarkers -Neopterin and CXCL-13- and we assessed their potential on the samples collected during post-treatment followup of patients. 2) determine new protein biomarkers using LC-MS/MS mass spectrometry. Our study identified a large number of potential biomarkers in cerebrospinal fluid, urine and saliva through the establishment of a new proteomic catalogue. Taking into account some of these proteins may improve patient management and follow-up in the future.
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Julien Bonnet. Exploitation d'une biobanque de patients atteints de Trypanosomose Humaine Africaine à Trypanosoma brucei gambiense : recherche et validation de biomarqueurs. Médecine humaine et pathologie. Université de Limoges, 2017. Français. ⟨NNT : 2017LIMO0117⟩. ⟨tel-02003614⟩

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