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, Lucas Philippe 1 , Ya-Zhuo Gong 1 , Seiamak Bahram 1, Jacques-Eric Gottenberg, vol.2, issue.1

I. Immunorhumatologie-moléculaire and . Umr-s_1109, France Abstract We evaluated micro (mi) RNA-mediated regulation of BAFF expression in fibroblasts using two concomitant models: (i) synovial fibroblasts (FLS) isolated from healthy controls (N) or Rheumatoid Arthritis (RA) patients; (ii) human dermal fibroblasts (HDF) isolated from healthy controls (N) or Systemic Sclerosis (SSc) patients. Using RT-qPCR and ELISA, we first showed that SScHDF synthesized and released BAFF in response to Poly(I:C) or IFN-c treatment, as previously observed in RAFLS, whereas NHDF released BAFF preferentially in response to IFN-c. Next, we demonstrated that miR-30a-3p expression was down regulated in RAFLS and SScHDF stimulated with Poly(I:C) or IFN-c. Moreover, we demonstrated that transfecting miR-30a-3p mimic in Poly(I:C)-and IFN-c-activated RAFLS and SScHDF showed a strong decrease on BAFF synthesis and release and thus B cells survival in our model. Interestingly, FLS and HDF isolated from healthy subjects express higher levels of miR-30a-3p and lower levels of BAFF than RAFLS and SScHDF. Transfection of miR-30a-3p antisense in Poly(I:C)-and IFNc-activated NFLS and NHDF upregulated BAFF secretion, confirming that this microRNA is a basal repressors of BAFF expression in cells from healthy donors. Our data suggest a critical role of miR, Centre National de Référence pour les Maladies Systémiques Autoimmunes Rares

G. Alsaleh, A. François, L. Philippe, Y. Gong, and S. Bahram, MiR-30a-3p Negatively Regulates BAFF Synthesis in Systemic Sclerosis and Rheumatoid Arthritis Fibroblasts, PLoS ONE, vol.9, issue.10, p.111266, 2014.

. Alsaleh, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited, 2014.

, Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files

, Jean Sibilia's work was supported by grants from Bristol Myers Squibb, Roche, Pfizer, Courtin Foundation and CAMPLP. Sébastien Pfeffer's work was supported by the European Research Council (ERC-StG-260767) and Agence Nationale pour la Recherche (labex netRNA, ANR-10-LABX-36). The funders had no role in study design, data collection and analysis

, Competing Interests: The authors have declared that no competing interests exist

, Email: galfarhan@unistra.fr . These authors contributed equally to this work

, L-glutamine, penicillin, streptomycin, amphotericin B, TRIzol reagent and DiOC 6 (3,39-Dihexyloxacarbocyanine Iodide) were from Invitrogen, LPS from Salmonella abortus equi and Propidium Iodide (PI) solution was obtained from Sigma Aldrich

, The miScript System, miRNA mimc and Allstars negative control siRNA were obtained from Qiagen (Courtabeuf, France). miR-30a-3p antagonists were from Fisher scientific (Illkirch Cedex, France), The enzyme immunoassay kits for human BAFF, APRIL and IL-6 detection and recombinant IFN-c were from R&D systems

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