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Molecular characterisation of the mechanism of target-dependant microRNA degradation

Abstract : Several regulatory mechanisms have been uncovered at every level of the biogenesis and the activity of miRNAs. However, there is less information about the regulation of the stability of miRNAs. The PhD project entailed the study of a process, which specifically enables the degradation of a cellular miRNA (miR-27) induced by a viral transcript (m169) during an infection by the mouse cytomegalovirus (MCMV). This miRNA is destabilized by a process called ‘target-RNA directed miRNA degradation’ (TDMD). I first undertook the study and the characterization of the molecular determinants and the cellular factors implicated in TDMD. Moreover, I started to set up a protocol in order to identify AGO2 partners of viral or host origin during MCMV infection, which would potentially be implicated in TDMD.
Keywords : MicroRNA TDMD MCMV BioID
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Semih Cetin. Molecular characterisation of the mechanism of target-dependant microRNA degradation. Genomics [q-bio.GN]. Université de Strasbourg, 2016. English. ⟨NNT : 2016STRAJ105⟩. ⟨tel-02003604⟩

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