Nouveaux concepts de revêtements antimicrobiens à base de peptides naturels et polypeptides appliqués aux dispositifs médicaux

Abstract : Nowadays, about half of hospital-acquired infections are due to medical devices implantation. In this context, we have developed two types of antimicrobial coatings adapted to the biomedical field. The first one is based on peptide composed of an anchoring sequence, an antimicrobial sequence and a pathogen-specific cleavage site and grafted on the substrate. The antimicrobial site will be released only in the presence of the pathogen through the use of the cleavage site. Despite of the success of peptide grafting, some parameters must be optimized in order to obtain an antimicrobial effect. The second antimicrobial coating concept is based on the layer-by-layer technique by using poly(L-arginine) (PAR) and hyaluronic acid (HA). The effect of the size of PAR chains on the antimicrobial character of the coating was investigated and it is proven that only films composed with PAR of 30 residues present an antibacterial effect. Moreover HA is the only polyanion leading to such antimicrobial multilayer. It is also demonstrated that this antimicrobial properties is maintained when other cationic homopolypeptides are used in association with HA in layer-by-layer films.
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Angela Mutschler. Nouveaux concepts de revêtements antimicrobiens à base de peptides naturels et polypeptides appliqués aux dispositifs médicaux. Chimie-Physique [physics.chem-ph]. Université de Strasbourg, 2017. Français. ⟨NNT : 2017STRAE025⟩. ⟨tel-02003597⟩

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