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Cyclic contractions contribute to 3D cell motility

Abstract : Cell motility is an important process in Biology. It is mainly studied on 2D planar surfaces, whereas cells experience a confining 3D environment in vivo. We prepared a 3D Cell Derived Matrix (CDM) labeled with fluorescently labeled fibronectin, and strikingly cells managed to deform the matrix with specific patterns : contractions occur cyclically with two contraction centers at the front and at the back of the cell, with a period of ~14 min and a phase shift of ~3.5 min. These cycles enable cells to optimally migrate through the CDM, as perturbation of cycles led to reduced motility. Acto-myosin was established to be the driving actor of these cycles, by using specific inhibitors. We were able to trigger cell motility externally with local laser ablations, which supports this framework of two alternating contractions involved in motion. Altogether, this study reveals a new mechanism of dynamic cellular behaviour linked to cell motility.
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Submitted on : Friday, February 1, 2019 - 12:20:21 PM
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  • HAL Id : tel-02003590, version 1



Amélie Godeau. Cyclic contractions contribute to 3D cell motility. Biological Physics []. Université de Strasbourg; Universität des Saarlandes, 2016. English. ⟨NNT : 2016STRAF038⟩. ⟨tel-02003590⟩



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