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Contribution des cellules souches de glioblastome à l'hétérogénéité tumorale : aspect thérapeutique et développement d'un système d'expression mosaïque fluorescent

Abstract : The glioblastoma multiforme (GBM) is the most aggressive primary brain tumor and includes a subpopulation of tumoral stem cells (CSG). Those cells can self-renew, proliferate and differentiate by expressing specific neural markers and/or transdifferentiate into vascular-like cells. In this context, my work consisted first to produce and characterize several CSG lines from patient biopsies to constitute a bank of cell lines with different properties. We also evaluated the impact of in house therapeutic transmembrane peptides targeting the neuropilin-1 / plexin-A1 receptor platforms overexpressed in GBM. We thus showed that both targeting peptides decrease the growth of GSC in in vitro and in vivo models. Finally, I developed an inducible mosaic expression system to track the live differentiation of CSG. This system is based on the expression of four different fluorescent reporters controlled by the activity of cell type specific promoters.
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Lionel Meyer. Contribution des cellules souches de glioblastome à l'hétérogénéité tumorale : aspect thérapeutique et développement d'un système d'expression mosaïque fluorescent. Biologie cellulaire. Université de Strasbourg, 2016. Français. ⟨NNT : 2016STRAJ109⟩. ⟨tel-02003526⟩

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