Skip to Main content Skip to Navigation
Theses

RNA/RNA interactions involved in the regulation of Benyviridae viral cicle

Abstract : Multipartite RNA virus condition to provide a complete set of genomic segments in each infected cell implies a high level of MOI that results in an unsustainable biological cost in terms of viral replication. In the proposed model, to minimize the cost of the genome integrity preservation, a network of RNA/RNA interactions determines the recognition and the mobilization of at least one of each genomic RNAs in a modular RNP complex. Such complex must be considered as the mobile infectious unit of the segmented genome during viral spread in the plant. The Aim of this thesis was to experimentally determine the existence of RNA/RNA interactions between BNYVV RNAs and their implication in the viral cycle. BNYVV genomic segments have been co-detected within isolated single cells from systemic tissues where they accumulate to reach set point genome formulas. In the model where vRNAs interact each other to form the minimal mobile infective unit, RNA1 and RNA2 interaction domain has been identified in silico and in vitro. The rationale of such an interaction has been provided in vivo using BNYVV and Beet soil-borne mosaic virus chimeras.
Complete list of metadatas

Cited literature [126 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02003448
Contributor : Abes Star :  Contact
Submitted on : Friday, February 1, 2019 - 11:55:00 AM
Last modification on : Thursday, September 24, 2020 - 3:23:18 AM
Long-term archiving on: : Thursday, May 2, 2019 - 5:21:47 PM

File

DALLARA_Mattia_2018_ED414.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02003448, version 1

Collections

Citation

Mattia Dall'Ara. RNA/RNA interactions involved in the regulation of Benyviridae viral cicle. Vegetal Biology. Université de Strasbourg; Università degli studi (Bologne, Italie), 2018. English. ⟨NNT : 2018STRAJ019⟩. ⟨tel-02003448⟩

Share

Metrics

Record views

109

Files downloads

141