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Self-assembly of ionic fluorescent dyes inside polymer nanoparticles : engineering bright fluorescence and switching

Abstract : Encapsulation of ionic dyes with help of bulky hydrophobic counterions into polymer nanomaterials emerged as powerful method for generating ultrabright fluorescent nanoparticles (NPs) for bioimaging. Here, this counterion-based approach is extended to cyanine dyes, operating from blue to near-infrared range. Based on cyanine-loaded NPs, a multicolour cell barcoding method for long-term cell tracking is developed. Second, the role of bulky hydrophobic counterion in self-assembly of cationic dyes inside polymeric NPs is studied by testing a large library of anions. We show that high hydrophobicity of a counterion enhances dye encapsulation, prevents particle aggregation and tunes dye clustering, while large size prevents dyes from self-quenching. Third, counterions based on aluminates and barbiturates are shown to outperform fluorinated tetraphenylborates. This work provides a solid basis for counterion-enhanced encapsulation and emission concept in preparation of dye-loaded fluorescent NPs.
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Bohdan Andreiuk. Self-assembly of ionic fluorescent dyes inside polymer nanoparticles : engineering bright fluorescence and switching. Other. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAF027⟩. ⟨tel-02003423⟩

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