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Ingénierie d'un outil basé sur une GFP fragmentée pour l'étude des protéines multi-localisées chez les eucaryotes

Abstract : Aminoacyl-tRNA synthetases catalyze aminoacyl-tRNA formation, required for protein synthesis but can also associate into multi-synthetase complexes (MSC). In S. cerevisiae, the AME complex contains glutamyl- and methionyl-tRNA synthetases bound to the anchor protein Arc1 and is responsible for the coordination of nuclear and mitochondrial genome expression. The three MSC partners are multi-localized and present simultaneously in several compartments. The detection of the organellar pools of these multilocalized proteins in vivo is difficult, since they are mainly cytosolic. Therefore, we engineered a split-GFP based localization tool that allows us to specifically visualize organellar fractions of multi-localized proteins. To do so, GFP was split into two parts: β1-10, restricted to a subcellular compartment and β11, fused to the protein of interest. This tool allowed us to study relocalization of cytosolic proteins and characterize targeting signals.
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Gaëtan Bader. Ingénierie d'un outil basé sur une GFP fragmentée pour l'étude des protéines multi-localisées chez les eucaryotes. Génomique, Transcriptomique et Protéomique [q-bio.GN]. Université de Strasbourg, 2017. Français. ⟨NNT : 2017STRAJ116⟩. ⟨tel-02003385⟩

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