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Study of the mechanisms of tRNA targeting into yeast and human mitochondria

Abstract : Mutations in the mitochondrial genome give rise to neurodegenerative diseases or myopathies. To develop gene therapy for preventing the appearance of these syndromes, we need to better understand the molecular mechanisms of mitochondrial RNA. For this purpose we try to recapitulate in vitro the import of RNA from cell extracts fractionated by different methods such as exclusion or affinity chromatography using tagged RNAs or proteins. Our results refine our knowledge of these mechanisms and allow to advance the idea that enolase, an enzyme of glycolysis, does not act alone during the first stage of import of tRNALys with anticodon CUU (tRK1). Indeed, we have shown that ultra-purified enolase no longer binds to tRK1 in vitro, while preparations of yeast mitochondria recapitulate the import when various fractions mixed with enolase were tested. The performed extracts fractionation make it possible to point to certain proteins which could work in concert with the enolase to convey tRK1 to mitochondria.
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Mariia Baleva. Study of the mechanisms of tRNA targeting into yeast and human mitochondria. Cellular Biology. Université de Strasbourg; Université Lomonossov (Moscou), 2016. English. ⟨NNT : 2016STRAJ096⟩. ⟨tel-02003364⟩

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