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Dispositifs microfluidiques pour l’injection de fluides à travers un réseau de gouttes : application biocapteur

Abstract : Since two decades the research on microfluidics systems allowed creating devices for biological detection with regular improvement in compactness, functionality integration and quantity of biological sample, leading to the concept of lab-on-chip. This approach has resulted in dramatic changes in the biomedical field, for example, opening the possibility to perform genomic analysis or improving the medical analysis cost. Using droplet as reaction chamber is a recent evolution that leads to a decrease in biological sample volume and an increase in analysis speed by multiplexing.Our team develops acoustical sensors dedicated to detect biomarker of interest in liquids. The principal weakness of theses sensors lies in their need for replacement of the biodetection interface for performing a new measurement. Accordingly, they use a detection chamber partially or totally disposable. However, few research works showed reusability of sensor by regenerating the bioreceptor layer on the detection interface by chemical treatment.We are proposing to avoid the replacement or the chemical treatment of the detection interface that requires dismounting the device between measurements. We are using here droplets, not as reaction chambers but as movable detection interface. They can be generated and configured directly inside the device to detect a specific biomarker. Then, droplets can be easily evacuated and replaced through the device, which allows to chain measurement of various configurations without dismounting it.The research work conducted in this thesis focuses on the fluidic aspects of this innovative sensor. They show development, including realization and characterization, of theses microfluidic devices and its dedicated characterization setup. This project is followed by two ancillary works about development of microfluidic devices for acoustical sensors and droplets systems. The first one is aiming at the homogenization of the flow velocity inside a reaction chamber. The second one is exploiting property of droplet generation for the realization of a variable capacitance capacitor.
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https://tel.archives-ouvertes.fr/tel-02001033
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Submitted on : Friday, February 1, 2019 - 1:08:44 PM
Last modification on : Wednesday, October 14, 2020 - 4:07:26 AM
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  • HAL Id : tel-02001033, version 1

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Charles-Louis Azzopardi. Dispositifs microfluidiques pour l’injection de fluides à travers un réseau de gouttes : application biocapteur. Autre. Université Bourgogne Franche-Comté, 2018. Français. ⟨NNT : 2018UBFCD020⟩. ⟨tel-02001033⟩

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