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Etude de l’effet antibactérien de surfaces traitées à l'aide de composés du titane et de leur applicabilité dans les industries agroalimentaires

Abstract : Biofilms in food industries represent a recurrent problem with economic and health safety implications. The development of photoactive surfaces with antibacterial properties could facilitate their elimination. One solution would be to deposit a thin layer of titanium dioxide (TiO2) on conventional materials used in food plants. The objectives of this thesis were the optimization of thin films of TiO2 with antibacterial properties and to study the mechanisms involved in photocatalysis. Deposits were made by radio-frequency sputtering under different conditions (temperature, oxygen partial pressure, duration), first on a glass substrate and then on 316 stainless steel. Characterization of thin layers by scanning microscopy, diffraction of X-ray, showed differences due to substrate changeTiO2 thin layers obtained either on glass or stainless steel showed photocatalytic and antibacterial properties on representative strains of the flora found in the meat industry: Listeria monocytogenes, Yersinia enterocolitica and Pseudomonas fragi. Decreases in the bacterial population between 1.5Log and 3Log were observed. The production of reactive oxygen species (ROS) was studied by spectrocolorimetric methods. It seems that H2O2 on the surface is converted in another ROS, ¿OH. Finally, the study of the bacterial response mechanisms to photocatalysis, especially for L. monocytogenes, was initiated by a transcriptomic approach by following the expression of genes involved in the response to oxidative stress. However, the method used requires an optimization in order to be used for the bac
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Marion Barthomeuf. Etude de l’effet antibactérien de surfaces traitées à l'aide de composés du titane et de leur applicabilité dans les industries agroalimentaires. Microbiologie et Parasitologie. Agrocampus Ouest, 2017. Français. ⟨NNT : 2017NSARB306⟩. ⟨tel-01996878⟩

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