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Développement d'une plateforme autonome et portable et pour des applications santé

Abstract : Microsystems utilizing microfluidic techniques offer the possibility to perform point-of-need biological analysis. An objective of these systems is to increase the efficiency, speed and accessibility of these analyses. In order to effectively develop this kind of device, a set of criteria must be established and adhered to. This set should address cost limitations, portability, user-friendliness, and accuracy of the results. Another objective is to propose a new portable system that has the capability to address as many applications as possible. To this end, complex biological assays with multiple steps and multiple reagents must be integrated and automated. ELISA is one such assay being considered.To deal with this issue, an innovative technique employs a hyper-elastic material joined to an X-Y architecture. The resulting chambers are flexible, thus allowing for calibration and mixing on the range of 1 µL to hundreds of µL. Several protocols are integrated and validated in microfluidic chips in order of increasing complexity. To start, a range of dilutions is performed, which is then used to calibrate biological assay. Next, an enzymatic assay and a homogeneous ELISA are integrated. Finally, heterogeneous ELISA, which is the aimed assay, is achieved.We present here a prototype to demonstrate the handling of the microfluidic chip. This platform is versatile and compatible with those that have been previously developed. Additionally, the introduction and integration of liquid reagents is proposed in order to completely automate the protocol.
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Submitted on : Monday, January 28, 2019 - 10:07:30 AM
Last modification on : Thursday, June 11, 2020 - 5:04:07 PM


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  • HAL Id : tel-01996023, version 1




Charlotte Parent. Développement d'une plateforme autonome et portable et pour des applications santé. Biophysique []. Université Grenoble Alpes, 2018. Français. ⟨NNT : 2018GREAY037⟩. ⟨tel-01996023⟩



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