Skip to Main content Skip to Navigation
Theses

Localisation de régions du génome du pommier contrôlant la variation de caractères de qualité du fruit et de résistance aux maladies : signatures de sélection et génétique d'association

Abstract : Since apple domestication, humans have progressively selected improved varieties, especially for traits linked with fruit quality, productivity or resistance to pathogens. The genetic bases underlying these traits have been explored thanks to genetic mapping in F1 segregating populations that only allows the study of a small part of the available genetic diversity. The aim of this work was to analyze the genetic bases of fruit quality and disease resistance against apple scab and fire blight, in collections of old apple varieties representing a much larger diversity. Genotyping of core collections was performed either with arrays of 8k and 480k SNPs or by resequencing of chosen genes. Signs of genetic differentiation were identified between cider and dessert apples and were partially linked to the polyphenols pathway. After studying linkage disequilibrium, both on a large and a small scale, an association genetics approach allowed the identification of genomic regions associated with the variation of several fruit quality traits. Especially, the top of linkage group 16 was found to be linked with acidity (locus Ma), firmness, juiciness and bitterness (LAR gene). Concerning the resistance of apple to fire blight, a region containing a homolog of the NPR1 gene (defense activator) was identified. This thesis allowed the refining of the putative localization of previously identified QTLs and the identification of new genetic resources that could be useful in future selection programs using marker assisted selection.
Document type :
Theses
Complete list of metadata

Cited literature [314 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01992546
Contributor : Abes Star :  Contact
Submitted on : Thursday, January 24, 2019 - 2:56:21 PM
Last modification on : Monday, July 6, 2020 - 3:40:00 PM

File

these_LEFORESTIER.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01992546, version 1

Citation

Diane Leforestier. Localisation de régions du génome du pommier contrôlant la variation de caractères de qualité du fruit et de résistance aux maladies : signatures de sélection et génétique d'association. Sciences agricoles. Université d'Angers, 2015. Français. ⟨NNT : 2015ANGE0051⟩. ⟨tel-01992546⟩

Share

Metrics

Record views

232

Files downloads

217