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Outils bioinformatiques pour l'analyse génétique de la résistance du moustique Anopheles gambiae vis-à-vis des parasites du paludisme

Abstract : During my PhD, I developed and implemented new methods and tools using the latest technologies of the Next Generation Sequencing, bioinformatics tools and the « reciprocal allele-specific RNA interference » (rasRNAi) method with the aim of identifying genetic and non-genetic factors responsible for the resistance of the mosquito Anopheles gambiae to the mouse malaria parasites Plasmodium berghei. I have implemented a strategy for identifying polymorphisms in the resistant and susceptible lines to (1) select genetic markers for future genetic analysis and (2) list the polymorphicgenes. I contributed to the development of a new allele-specific dsRNA probe for the rasRNAi method by identifying how mosquitoes process the injected dsRNA by the analysis of sequenced small RNAs from the injected dsRNA. I developed a pipeline to identify the microbiota composition in susceptible and resistant lines in order to compare them.
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https://tel.archives-ouvertes.fr/tel-01988755
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Submitted on : Tuesday, January 22, 2019 - 3:10:41 AM
Last modification on : Tuesday, September 15, 2020 - 4:15:15 PM
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Anaïs Vittu. Outils bioinformatiques pour l'analyse génétique de la résistance du moustique Anopheles gambiae vis-à-vis des parasites du paludisme. Bio-Informatique, Biologie Systémique [q-bio.QM]. Université de Strasbourg, 2015. Français. ⟨NNT : 2015STRAJ096⟩. ⟨tel-01988755⟩

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