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Réalisation d'une pince acoustofluidique pour la manipulation de bioparticules

Abstract : In lab-on-a-chip (LOC) technologies, many sample preparation steps are required before achieving a biological analysis on a single chip (sample introduction, concentration, mixing, purification, separation, etc.). The microsystem team of the Ampere Lab has studied for many years different contactless particle manipulation techniques, for sorting or manipulating bioparticles in LOC platforms, such as dielectrophoresis and magnetophoresis. In this thesis, we focus on acoustic manipulation of microparticles. This technique is advantageous for the manipulation of biological objects such as bacteria, because labelling and medium exchange can be avoided. We chose to work with surface acoustic waves (SAW), because this approach is consistent with the use of PDMS, widely used in microfluidics. Besides an easier microfluidic integration of the acoustic tweezers, the SAW technology provides an alternative to the existing devices with fixed acoustic traps, allowing a real time control of the trapped particles. This was experimentally achieved by playing on the phase shift between the two electrical signals driving the IDT, thereby modifying the position of nodes and antinodes of the resulting pressure wave. As a result, we could control in real time the position of a 3 μm latex bead or an E.coli bacteria alignment. We have also developed a finite-element model of the whole acoustofluidic chip allowing a better understanding of the physics and the optimization of the energy transfer between the electrical source and the trapped particle. Among different results, this model informs us that the magnitude of the acoustic radiation force varies by a factor of two with the phase shift between the electrical sources. This result seems to be validated by our last experiments.
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  • HAL Id : tel-01970558, version 1

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Sylvain Toru. Réalisation d'une pince acoustofluidique pour la manipulation de bioparticules. Micro et nanotechnologies/Microélectronique. Ecole Centrale de Lyon, 2014. Français. ⟨NNT : 2014ECDL0028⟩. ⟨tel-01970558⟩

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