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Muscle to epidermis mechanotransduction’ pathways involved in C. elegans embryonic elongation

Abstract : C. elegans embryonic elongation is driven by 2 forces: Actomyosin contractility and Muscle contraction (after 1.7-fold). Actomyosin contraction is regulated by the Rho kinase and the serine/threonine p21 activated kinase pak-1. Tension provided by muscle contraction recruits GIT-1 to hemidesmosomes (HD), which in turn facilitates further elongation by activating proteins such as PAK-1 (Nature 2011). Surprisingly in absence of git-1/pak-1, elongation still continues, which led us to hypothesize parallel pathways. An RNAi screen was performed to get the candidates in the parallel pathway/s. Candidates interacting strongly with git-1 belonged to the Dynein Dynactin complex. By use of temperature sensitive alleles and microtubule severing proteins, we found a microtubule independent role of Dynactin in epidermis and that dynactin functionally interacts with spectraplakin vab-10 and spectrin spc-1, which allows us to portray the role of Dynein Dynactin complex during embryonic elongation.
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Saurabh Tak. Muscle to epidermis mechanotransduction’ pathways involved in C. elegans embryonic elongation. Development Biology. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAJ054⟩. ⟨tel-01967617⟩

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