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Caractérisation de la protéine 140K impliquée dans l’adressage aux chloroplastes des complexes de réplication du virus de la mosaïque jaune du navet (TYMV)

Abstract : Turnip yellow mosaic virus (TYMV) is a positive single-stranded RNA virus. Among the three ORFs encoded by the TYMV genome, 206K is the only protein required for viral replication. It is cleaved into 140K and 66K, which are both present at the chloroplast envelope membrane, where viral replication takes place.The 140K protein is targeted to chloroplasts, where it recruits 66K, and behaves as an integral membrane protein. The chloroplast targeting domain (DAC) of the 140K protein was defined using Arabidopsis thaliana protoplasts transfected by various constructs encoding deleted versions of 140Kfused to EGFP and subsequent confocal microscopy. The DAC comprises two amphipathic alpha helices, as confirmed by circular dichroism. Their involvement in chloroplast localisation and membrane association has been assessed, as well as their contribution to viral replication.We observed different subcellular distribution patterns of 140K protein, which correlate with the expression level of the protein. Its capability to dimerize has also been demonstrated.The involvement of other DAC residues in subcellular localisation, dimerization and viral replication has been studied.
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Lucille Moriceau. Caractérisation de la protéine 140K impliquée dans l’adressage aux chloroplastes des complexes de réplication du virus de la mosaïque jaune du navet (TYMV). Sciences agricoles. Université Paris Saclay (COmUE), 2015. Français. ⟨NNT : 2015SACLS255⟩. ⟨tel-01967604⟩

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