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Flow of healthy and sickle red blood cells in microcirculatory conditions : clustering process and self-margination phenomenon

Abstract : I experimentally characterized the clustering formation of healthy and sickle red blood cells (RBCs) flowing through straight micro-capillaries. The effect of aggregation was also investigated. I found that cluster formation under physiological conditions is most likely caused by a combination of hydrodynamic and macromolecule-induced interactions. Macromolecule-induced interactions are not fully overcome by shear stresses within the physiological range, and they contribute to cluster stability. Moreover, I found that a pronounced bimodal distribution of the cell-to-cell distances in the hydrodynamic clusters is produced.Additionally, I investigated experimentally the collective behavior of oxygenated sickle RBCs and their distribution along cylindrical micro-capillaries with diameters comparable to a human venule or arteriole. I have shown that there is a heterogeneous distribution of RBCs according to their density: low-density cells tend to stay closer to the center of the channel, while most dense cells (also more rigid) self-marginated under defined conditions. Aggregation seems to inhibit self-margination depending on the aggregative factor and patient: dextran allows self-margination in some patients and inhibits it in others. Plasma inhibits self-margination of cells in all cases, highlighting the importance of the plasma proteins and adhesive molecules in the aggregation phenomena.
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Submitted on : Friday, December 14, 2018 - 3:36:21 PM
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Viviana Andrea Claveria Pizarro. Flow of healthy and sickle red blood cells in microcirculatory conditions : clustering process and self-margination phenomenon. Other [cond-mat.other]. Université Montpellier; Universität des Saarlandes, 2017. English. ⟨NNT : 2017MONTS081⟩. ⟨tel-01955750⟩

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