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Theses

Nouvelles stratégies d'amplification moléculaire d'un signal basées sur l'activation de dérivés pro-quinoniques : de l'activation d'un catalyseur biomoléculaire au déclenchement d'une réaction auto-catalytique

Abstract : Generally, diagnosing a given pathology at an early stage of development promotes the patient's prognosis. Such a performance requires the detection of specific markers which are present in complex biological fluids at low concentration level. To detect these extremely low analyte concentrations, the strategy employed in this work is the molecular amplification of the signal. To this end, different approaches are possible (i) amplifying the signal resulting from the target / probe recognition event, (ii and iii) amplifying the signal by regeneration or replication of the target. The strategies conceived during this thesis work mainly focus on the detection of small molecules, such as hydrogen peroxide or fluoride anion, but with the idea of extending them to the indirect detection of biomarkers or proteins of interest. The first part of this thesis focuses on the molecular amplification of a signal by allosteric catalysis using the reconstitution reaction of a given apoenzyme with its cofactor. The second part of this thesis is based on the implementation of catalytic and auto-catalytic amplification systems for the detection of H2O2, thanks to pro-quinonic derivatives bearing boronic acid/ester group. The distinction between catalytic and auto-catalytic systems is based on whether H2O2 is regenerated or amplified during the reaction.
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https://tel.archives-ouvertes.fr/tel-01952830
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Submitted on : Wednesday, December 12, 2018 - 2:13:06 PM
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  • HAL Id : tel-01952830, version 1

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Charlie Rabin. Nouvelles stratégies d'amplification moléculaire d'un signal basées sur l'activation de dérivés pro-quinoniques : de l'activation d'un catalyseur biomoléculaire au déclenchement d'une réaction auto-catalytique. Autre. Université Sorbonne Paris Cité, 2017. Français. ⟨NNT : 2017USPCC079⟩. ⟨tel-01952830⟩

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