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Traitement des pathologies cornéennes par thérapie cellulaire et bioingénierie tissulaire

Abstract : Cornea, the outer part of the eye, features high transparency crucial for good vision. The maintenance of a healthy cornea is linked to the presence of corneal stem cells in the limbus (peripheral region of the cornea). Several diseases can lead to its opacification requiring transplantation of donor tissue to restore vision. Although corneal transplantation has achieved clinical success, there is a shortage of donor corneas worldwide. To solve this problem, cell therapy and tissue-engineered artificial cornea are promising approaches that could eventually outperform current treatment. Corneal epithelial stem cells (LSC) and stromal stem cells (SSC) were isolated in feeder free and xeno free medium. The therapeutic effect of SSC was investigated in vivo by their injection in mouse cornea treated with liquid nitrogen. SSC have the ability to restore the extracellular matrix organization and corneal transparency. Transparent collagen I fibrillated matrices have been synthesized and were evaluated for fibril organization, transparency, mechanical properties and their ability to be repopulated by corneal stromal stem cells and to support limbal epithelial stem cell growth. We show that the matrices were organized and transparent. SSC and LSC were able to repopulate and to grow into the collagen matrix. These cells present a potential for stem cell-based treatment of corneal blindness.
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Submitted on : Monday, December 10, 2018 - 1:02:37 AM
Last modification on : Friday, May 29, 2020 - 4:02:48 PM
Long-term archiving on: : Monday, March 11, 2019 - 12:46:50 PM


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  • HAL Id : tel-01949307, version 1


Djida Ghoubay-Benalloua. Traitement des pathologies cornéennes par thérapie cellulaire et bioingénierie tissulaire. Biologie cellulaire. Université Pierre et Marie Curie - Paris VI, 2017. Français. ⟨NNT : 2017PA066445⟩. ⟨tel-01949307⟩



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