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, Dans un même temps, des mélanges contenant 1,5 % (m/v) chitosane (poudre) sont préparés (V = 6,6 mL)
, Après l'activation du PNIPAM-COOH, les mélanges contenant le chitosane sont ajou
, g/mol) pour éliminer le PNIPAM-COOH n'ayant pas réagi. L'eau de dialyse est changée trois fois par jour. Après dialyse, les échantillons sont lyophilisés pendant 3 jours
, De l'alginate (poudre) est mis dans 5 mL de trois solvants différents (H 2 O, un tampon et DMSO) contenant NHS (860 µmol, 1 eq) et EDC (1 600 µmol
40 ou 200 eq) est mélangée à 5 mL de solvant, vol.2 ,
, g/mol) pour éliminer la diamine n'ayant pas réagi. L'eau de dialyse est changée trois fois par jour. Après dialyse, les échantillons d'alginate-NH 2 obtenus sont lyophilisés pen
ème étape Des mélanges contenant le PNIPAM-COOH (Mn = 5 000 g/mol, 150 mg, 1 eq), NHS (90 µmol, 3 eq) et EDC (130 µmol, 4 eq) sont préparés pour activer l'acide carboxylique, p.5 ,
, Trois solvants sont utilisés : H 2 O, un tampon et DMSO. Le tampon est une solution
, Dans un même temps, des mélanges contenant 1,5 % (m/v) d'alginate-NH 2 (issus de la première étape) sont préparés dans différents solvants (V = 10 mL) : H 2 O, un tampon et DMSO. Le tampon est une solution tamponnée PBS (0,01 M)
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, for disease treatment. These biomaterials have to fulfil various requirements depending on the application but in all cases biocompatibility is a crucial point that has to be considered in early processing stages. Biocompatibility is defined as the ability to act in a living system without any toxicity or rejection, whether physiological or immunological. Although biocompatibility includes "non-toxicity, E-mail address: audrey.tourrette@univ-tlse3.fr (A. Tourrette)
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