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G. Wishart, Un double développement a permis de séparer les lipides neutres. Lors de la première migration, un mélange hexane / diéthyl éther / acide acétique (20/5/0.5, v:v:v) a été utilisé et un mélange hexane / diéthyl éther (93/3, v:v) lors de la deuxième. Les lipides polaires ont eux été séparés après un seul développement, en utilisant, comme solvant d'élution, Sites internet: Site animal cell biology, vol.71, pp.113-118, 1984.

, Six classes de lipides neutres (alcools, acides gras libres, esters de stérols, éthers de glycérides, triglycérides et stérols libres) et sept classes de lipides polaires (phosphatidylethanolamine, phosphatidylcholine, phosphatidylsérine, phosphatidylinositol, cardiolipide, céramide aminoéthylphosphonate et lysophosphatidylcholine)

, La quantification a été réalisée par étalonnage externe, par densitométrie, à l'aide d'un scanner réglé à 370 nm et du logiciel Wincats (CAMAG)

, Résultats Aucune différence dans le contenu en lipides neutres et polaires du spermatozoïde d'huître creuse n'a été mesurée entre 5h et 12h post activation dans l

, Lipides neutres et polaires du spermatozoïde d'huître creuse mesurés entre 5h et 12h post activation dans l'eau de mer. TG : triglycéride, ST : stérols libres, ALC : alcools, PE : phosphatidylethanolamine, PI : phosphatidylinositol, CL: cardiolipide, PS: phosphatidylsérine, PC : phosphatidylcholine, CAEP : céramide aminoéthylphosphonate, vol.20

, Annexe 3

, Liste des publications et participation aux congrès Chapitre

M. Demoy-schneider, N. Schmitt, M. Suquet, C. Labbé, M. Boulais et al., Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the black-lip pearl oyster, Pinctada margaritifera, Spermatozoa: Biology, Motility and Function and Chromosomal abnormalities, pp.15-74
URL : https://hal.archives-ouvertes.fr/hal-01205080

M. Boulais, P. Soudant, L. Goïc, N. Quéré, C. Boudry et al., Individual variability of cellular and biochemical characteristics of Pacific oyster (Crassostrea gigas) spermatozoa: involvement in fertilization success, Prep

M. Boulais, G. Simon, C. Quéré, M. Suquet, P. Boudry et al., H and 31 P-Nuclear Magnetic Resonance analyses of metabolic changes of Pacific oyster (Crassostrea gigas) spermatozoa during the movement phase, Prep

M. Boulais, L. Goïc, N. Suquet, M. Quéré, C. Boudry et al., Respective roles of glycolysis and oxidative phosphorylation in ATP production for spermatozoon motility in the Pacific oyster, Crassostrea gigas, Prep

M. Boulais, P. Soudant, L. Goïc, N. Quéré, C. Boudry et al., Changes of cellular, biochemical and movement parameters during the motility phase of Pacific oyster, Crassostrea gigas, spermatozoa, Prep

M. Boulais, C. Corporeau, A. Huvet, I. Bernard, C. Quere et al., Assessment of oocyte quality in Pacific oyster, Crassostrea gigas

L. Goïc, N. Hegaret, H. Boulais, M. Beguel, J. P. Lambert et al., Flow cytometry to assess morphology, viability and production of oxygen reactive species of Crassostrea gigas oocytes. Application to toxic dinoflagellate Alexandrium minutum exposure, Accepté dans Cytometry, Part A

M. Suquet, C. Labbé, S. Puyo, C. Mingant, B. Quittet et al., Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas), PLoS One, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01205087

C. Corporeau, G. Vanderplancke, M. Boulais, M. Suquet, C. Quéré et al., Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas, J Proteomics, vol.75, issue.18, 2012.
URL : https://hal.archives-ouvertes.fr/hal-00788236

M. Suquet, J. Cosson, A. Donval, C. Labbe, M. Boulais et al., Marathon vs sprint racers: an adaptation of sperm characteristics to the reproductive strategy of Pacific oyster, turbot and seabass, J. Appl. Ichthyol, vol.28, 2012.
URL : https://hal.archives-ouvertes.fr/hal-00766671

M. Demoy-schneider, N. Schmitt, M. Suquet, C. Labbé, M. Boulais et al., Biological characteristics of sperm in two oyster species: the Pacific oyster (Crassostrea gigas) and the black-lip pearl oyster (Pinctada margaritifera). Oral presentation at the congress, Aquaculture Europe, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01205080

R. Sussarellu, P. Soudant, C. Lambert, C. Fabioux, I. Paul-pont et al., Experimental long term exposure of microplastics affects oyster reproduction. Oral presentation at the congress "Physiomar, 2014.

M. Boulais, L. Goïc, N. Suquet, M. Quéré, C. Boudry et al., The role of mitochondria in energy production for Pacific oyster (Crassostrea gigas) sperm motility. Oral presentation at the congress of the, Society for Experimental Biology Annual Meeting, 2014.

M. Boulais, L. Goïc, N. Suquet, M. Quéré, C. Boudry et al., ATP as a key factor for Pacific oyster (Crassostrea gigas) sperm movement and fertilization capacity. Oral presentation at the congress, National Shellfisheries Association, 2014.

J. P. Béguel, M. Boulais, M. Mercier, C. Lambert, A. Huvet et al., Antioxidant protection of intra-gonadic and free gametes of the Pacific oyster Crassostrea gigas. Oral presentation at the congress, National Shellfisheries Association, 2014.

M. Suquet, M. Arsenault-pernet, L. Goïc, N. Soudant, P. Mingant et al., Microplastics are love-killers for Pacific oysters! Oral presentation at the congress "Fate and impact of microplastics in marine ecosystems, 2014.

M. Boulais, L. Goïc, N. Suquet, M. Quéré, C. Boudry et al., Biological and energetic characteristics of sperm of Pacific oyster (Crassostrea gigas) in relation to its fertilization capacity. Oral presentation at the congress, Biology of Fish Gametes, 2013.

M. Suquet, C. Labbé, S. Puyo, B. Quittet, C. Mingant et al., Thawed Pacific oyster (Crassostrea gigas) larvae grow and reproduce at the same rate as fresh ones. Oral presentation at the congress, Biology of Fish Gametes, 2013.

M. Suquet, C. Quéré, M. Boulais, C. Mingant, L. Lebrun et al., Effects of maturation process and time post activation on sperm characteristics in scallop (Pecten maximus). Oral presentation at the congress "PHYSIOMAR12, 2012.

M. Suquet, M. Boulais, C. Corporeau, A. Huvet, C. Quéré et al., Quality criteria of Pacific oyster oocytes. Oral presentation at the congress "PHYSIOMAR10, 2010.

M. Boulais, B. Dzyuba, M. Suquet, E. J. Arsenault-pernet, F. Malo et al., Effect of pH on activation and movement regulation of sperm in the Pacific oyster (Crassostrea gigas). Poster presentation at the congress "Physiomar, 2014.

S. Madec, G. Vanderplancke, M. Boulais, M. Suquet, C. Quéré et al., Proteomic identification of quality factors in the oocyte of Pacific oyster Crassostrea gigas. Poster presentation at the, European Protomic Association, 2012.

F. Rimond, M. Boulais, F. Henaff, C. Fauvel, W. Leedy et al., Image J casa: A tool to assess the swimming characteristics of various cell types. Poster presentation at the, European Aquaculture Society, pp.1110-1111, 2010.