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, Chez l'Homme, la production des deux sous-unités ribosomiques débute dans le nucléole par la synthèse par l'ARN polymérase I d'un long transcrit contenant les séquences des ARN ribosomiques 5.8S, 18S et 25S, qui s'associe de manière co-transcriptionnelle à des protéines ribosomiques et à des facteurs d'assemblage. Le quatrième ARN ribosomique, l'ARNr 5S est transcrit séparément par l'ARN polymérase III, et s'associe avec les protéines ribosomiques Rpl5 et Rpl11 en dehors du ribosome. Ce sous-complexe, appelé particule 5S, est ensuite intégré au sein de la grande sous-unité. La particule 5S est également impliquée dans le contrôle de la prolifération cellulaire. En effet, en cas de dé-régulation de la biogenèse du ribosome, RÉSUMÉ La biogenèse des ribosomes est un processus complexe qui implique la production et l'assemblage de 4 ARN et d'environ 80 protéines

, Ces protéines assurent deux fonctions distinctes : elles sont requises pour l'association de la particule 5S avec la sous-unité pré-60S, et stimulent la transcription des ARNr par l'ARN polymérase I. Elles sont donc impliquées dans deux événements fondamentaux qui conditionnent les capacités de prolifération cellulaire, L'objectif principal de ma thèse est d'étudier le rôle des facteurs d'assemblage Rpf2 et Rrs1 dans la biogenèse du ribosome