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Theses

Intérêt du traitement par UV-C des communautés bactériennes, fongiques et des protistes autotrophes des biofilms colonisant la pierre patrimoniale : structure des peuplements, effets des UV-C sur la physiologie algale et innocuité du traitement vis à vis du support pictural.

Abstract : Development of biofilm on monument heritage are often considered as agents leading to aesthetic issues and biodeterioration of their support. The aim of this study is the use of UV-C light as alternative treatment against Lampenflora. Bacterial, algal and fungal communities were studied using new generation sequencing approach. Following UV-C treatments, physiological responses of Chlorella sp. were studied in laboratory (cell death, chlorophyll degradation, photosystem II status, etc.). Damages at cellular and molecular levels (cell death, bleaching of the chlorophyll) were taken into consideration to optimize the UV-C treatment. Then, to ensure the safety of UV-C for biofilms support (parietal paintings), several pigments and binders, used by prehistoric human, were highly irradiated. The results showed that UV-C radiations were not deleterious for inorganic compounds, while a color change was observed on organic binders. Finally, UV-C treatment has proven its effectiveness in La Glacière show cave by eradicating all biofilms. In addition, no recolonization was observed two years after treatment, showing that this treatment, which is the most environmental friendly to date, is effective over time.
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https://tel.archives-ouvertes.fr/tel-01905921
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Submitted on : Friday, October 26, 2018 - 11:39:06 AM
Last modification on : Monday, October 12, 2020 - 8:46:06 AM
Long-term archiving on: : Sunday, January 27, 2019 - 1:37:18 PM

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  • HAL Id : tel-01905921, version 1

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Stéphane Pfendler. Intérêt du traitement par UV-C des communautés bactériennes, fongiques et des protistes autotrophes des biofilms colonisant la pierre patrimoniale : structure des peuplements, effets des UV-C sur la physiologie algale et innocuité du traitement vis à vis du support pictural.. Sciences agricoles. Université Bourgogne Franche-Comté, 2017. Français. ⟨NNT : 2017UBFCD030⟩. ⟨tel-01905921⟩

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