A. Komla and G. , Etude de la structure des gels protéiques par Microscopie confocale, 2010.

P. Aymard, D. Durand, and T. Nicolai, The effect of temperature and ionic strength on the dimerisation of ?-lactoglobulin, International Journal of Biological Macromolecules, vol.19, pp.213-221, 1996.

P. Aymard, D. Durand, and T. Nicolai, A Comparison of the Structure of ?Lactoglobulin Aggregates Formed at pH7 and pH2. Int, Journal Polymer Analysis & Characterization, vol.2, pp.115-119, 1995.

R. A. Badley, D. Atkinson, H. Hauser, and D. Oldani, The structure, physical and chemical properties of the soy bean protein glycinin, Biochimica et Biophysica Acta, vol.412, pp.214-228, 1975.

H. A. Barnes, A handbook of elementary rheology. The University of Wales Institute of Non-Newtonian Fluid Mechanics, pp.119-139, 2000.

J. R. Brooks and C. V. Morr, Current aspects of soy protein fractionation and nomenclature, Journal of the American Oil Chemists' Society, vol.62, pp.1347-1348, 1985.

C. M. Bryant and D. J. Mcclements, Molecular basis of protein functionality with special consideration of cold-set gels derived from heat-denatured whey, vol.9, pp.143-151, 1998.

P. Cayot and D. Lorient, Structures et technofonctions des protéines du lait, 1998.

N. Chen, M. Zhao, C. Chassenieux, and T. Nicolai, Thermal aggregation and gelation of soy globulin at neutral pH, Food Hydrocolloids, vol.61, pp.740-746, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01927374

N. Chen, M. Zhao, F. Niepceron, T. Nicolai, and C. Chassenieux, The effect of the pH on thermal aggregation and gelation of soy proteins, Food Hydrocolloids, vol.66, pp.27-36, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01926821

R. Colsenet, O. S. Derman, and F. Mariette, Pulsed field gradient NMR study of Poly(ethylene glycol) Diffusion in Whey Protein Solutions and Gels, Macromolecules, vol.39, pp.1053-1059, 2006.

J. Conchello and J. W. Lichtman, Optical sectioning microscopy, vol.2, pp.920-931, 2005.

P. Croguennoc, T. Nicolai, M. E. Kuil, and J. G. Hollander, Self-diffusion of native proteins and dextran in heat-set globular protein gels, J. Phys. Chem. B, vol.105, pp.5782-5788, 2001.

T. Croguennec, D. Mollé, R. Mehra, and S. Bouhallab, Spectroscopic characterization of heat-induced non native ?-lactoglobulin monomers, Protein science, vol.13, pp.1340-1346, 2004.

. Chapitre-i-bibliographie,

H. Deschout, J. Hagman, and S. Fransson, Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope, Optics express, 2010.

G. Foffi, F. Sciortino, and P. Tartaglia, Structural arrest in dense star-polymer solutions, 2003.

P. Fox and D. Lorient, Developments in dairy chemistry, pp.145-239, 1989.

M. J. Garcia-salinas, F. J. De-las, and N. , A study of the primary electroviscous effect in monodisperse carboxyl polystyrene latex suspensions, Progress in Colloid & Polymer Science, vol.110, pp.134-138, 1998.

D. B. Genovese, Shear rheology of hard-sphere, dispersed, and aggregated suspensions and filler-matrix composites, Advances in Colloid and Interface Science, 2012.
DOI : 10.1016/j.cis.2011.12.005

D. George and J. Phillies, Dynamics of polymers in concentrated solutions: The Universal Scaling Equation Derived, Macromolecules, vol.20, pp.558-564, 1987.

J. Guo, . Xiao-quan, X. Q. Yang, and X. T. He, Limited aggregation behavior of betaconglycinin and its terminating effect on glycinin aggregation during heating at pH 7.0, Journal of Agricultural and Food Chemistry, vol.60, pp.78-85, 2012.
DOI : 10.1021/jf300409y

X. Gu, L. J. Campbell, and S. R. Euston, Effects of different oils on the properties of soy protein isolate emulsions and gels, Food Research International, vol.42, pp.925-932, 2009.

S. G. Hambling, A. S. Mc-alpine, and L. Sawyer, 1???). ?-lactoglobulin, Advanced dairy chemistry, pp.141-190

C. M. Hollar and N. Parris, Factors affecting the denaturation and aggregation of whey proteins in heated whey protein concentrate mixtures, Journal Dairy Science, vol.78, pp.260-267, 1995.

J. M. Jung and G. Savin, Structure of heat-induced ?-Lactoglobulin aggregates and their complexes with Sodium-Dodecyl Sulfate, Biomacromolecules, vol.9, pp.2477-2486, 2008.
DOI : 10.1021/bm800502j

J. E. Kinsella and D. M. Whitehead, Proteins in whey: chemical, physical and functional properties, Advances in food and nutrition research, vol.33, pp.343-438, 1989.
DOI : 10.1016/s1043-4526(08)60130-8

C. Le-bon, D. Durand, and T. Nicolai, Influence of genetic variation on the aggregation of heat-denatured ?-lactoglobulin, International Dairy Journal, vol.12, pp.671-678, 2002.

C. Le-bon and T. Nicolai, Self-Diffusion and Cooperative Diffusion of Globular Proteins in Solution, J. Phys. Chem, vol.103, pp.10294-10299, 1999.

N. Loren, M. Nyden, and A. M. Hermansson, Determination of local diffusion properties in heterogeneous biomaterials, Advances in Colloid and Interface Science, vol.150, pp.5-15, 2009.

. Chapitre-i-bibliographie,

J. S. Mackie and P. Meares, The sorption of electrolytes by a cation-exchange resin membrane. The royal society of London, series A, Mathematical and Physical Sciences, vol.232, pp.485-498, 1955.

N. Mahmoudi, S. Mehalebi, T. Nicolai, D. Durand, and A. Riaublanc, Light scattering study of the structure of aggregates and gels formed by heat-denatured whey protein isolate and ?-lactoglobulin at neutral pH, Journal of Agricultural and Food Chemistry, vol.55, pp.3104-3111, 2007.

R. Majhi, R. Ganta, and P. Vanam, Electrostatically Driven Protein Aggregation? ?Lactoglobulin at Low Ionic Strength, Langmuir

N. Maruyama, M. Adachi, and K. Takahashi, Crystal structures of recombinant and native soybean ?-conglycinin ? homotrimers, Eur. J. Biochem, vol.68, pp.5-5, 2001.

L. Masaro and X. X. Zhu, Physical models of diffusion for polymer solutions, gels and solids. Progress in polymer science, vol.24, pp.731-775, 1999.

S. Mehalebi, T. Nicolai, and D. Durand, Light scattering study of heat denatured globular protein aggregates, International Journal of Biological Macromolecules, vol.43, pp.129-135, 2008.

T. Nicolai, Formation and functionality of self-assembled whey protein microgels, Colloids and surfaces B : Biointerfaces, vol.137, pp.32-38, 2015.

T. Nicolai, M. Britten, and C. Schmitt, ?011). ?-lactoglobulin and WPI aggregates: formation, structure and applications, Food hydrocolloids, vol.25, pp.1945-1962

T. Nicolai and D. Durand, Controlled food protein aggregation for new functionality, Current Opinion in Colloid & Interface Science, vol.18, pp.249-256, 2013.

R. P. Nze, T. Nicolai, C. Chassenieux, and E. Nicol, Effect of connectivity on the structure and the liquid?solid transition of dense suspensions of soft colloids, Macromolecules, vol.48, pp.7-12, 2015.

T. Phan-xuan, D. Durand, and T. Nicolai, On the crucial importance of the pH for the formation and self-stabilization of protein microgels and strands, Langmuir, vol.27, pp.15092-15101, 2011.

D. Renard, J. Lefebvre, M. C. Griffin, and W. G. Griffin, Effects of pH and salt environment on the association of ?-lactoglobulin revealed by intrinsic fluorescence studies, International Journal of Biological Macromolecules, vol.22, pp.41-49, 1998.

C. Schmitt, C. Bovay, A. M. Vuilliomenet, M. Rouvet, L. Bovetto et al., Multiscale characterization of individualized ?-lactoglobulin microgels formed upon heat treatment under narrow pH range conditions, Langmuir, vol.25, pp.7899-7909, 2009.

J. Schweizer and . Ks, Glassy dynamics and mechanical response in dense fluids of soft repulsive spheres. I. Activated relaxation, kinetic vitrification, and fragility, The Journal of chemical physics, vol.134, 2011.

C. Tsenoglou, Rubber elasticity of cross-linked networks with trapped entanglements and dangling chains, Macromolecules, vol.22, pp.284-289, 1989.

D. Vlassopoulos and M. Cloitre, Tunable rheology of dense soft deformable colloids, Current Opinion in Colloid & Interface Science, vol.19, pp.561-574, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01223839

D. Vlassopoulos and G. Fytas, Frompolymers to colloids: engineering the dynamic properties of hairy particles, pp.1-54, 2009.

. Chapitre-ii-matériaux and . Méthodes,

I. I. Chapitre, Matériaux et Méthodes

T. Nicolai, M. Britten, and C. Schmitt, Food Hydrocolloids, 1945.

R. Mezzenga and P. Fischer, Reports on Progress in Physics, p.46601, 2013.

J. C. Gimel, D. Durand, and T. Nicolai, Macromolecules, vol.27, pp.583-589, 1994.

N. Mahmoudi, S. Mehalebi, T. Nicolai, D. Durand, and A. Riaublanc, J. Agric. Food Chem, vol.55, pp.3104-3111, 2007.

T. Hagiwara, H. Kumagai, and K. Nakamura, Bioscience, biotechnology, and biochemistry, vol.60, pp.1757-1763, 1996.

N. Micali, V. Villari, M. A. Castriciano, A. Romeo, and L. M. Scolaro, The Journal of Physical Chemistry B, vol.110, pp.8289-8295, 2006.

R. Vreeker, L. Hoekstra, D. D. Boer, and W. Agterof, Food Hydrocolloids, vol.6, pp.423-435, 1992.

M. Weijers, R. W. Visschers, and T. Nicolai, Macromolecules, vol.35, pp.4753-4762, 2002.

L. Donato, C. Garnier, J. L. Doublier, and T. Nicolai, Biomacromolecules, vol.6, pp.2157-2163, 2005.

D. B. Genovese, Advances in Colloid and Interface Science, pp.171-172, 2012.

D. Quemada and C. Berli, Advances in Colloid and Interface Science, vol.98, pp.51-85, 2002.

F. Sciortino and P. Tartaglia, Advances in Physics, vol.54, pp.471-524, 2005.

D. Vlassopoulos and M. Cloitre, Current Opinion in Colloid & Interface Science, vol.19, pp.561-574, 2014.

, Soft Matter, vol.12, pp.2785-2793, 2016.

T. Aubry, B. Largenton, and M. Moan, Journal of colloid and interface science, vol.202, pp.551-553, 1998.

I. R. Collins, Journal of Colloid and Interface Science, vol.178, pp.361-363, 1996.

C. Tsenoglou, J. Rheol, vol.34, pp.15-24, 1990.

N. Kovalchuk, I. Kuchin, V. Starov, and N. Uriev, Colloid Journal, vol.72, pp.379-388, 2010.

K. Baussay, C. Le-bon, T. Nicolai, D. Durand, and J. Busnel, International Journal of biological Macromolecules, vol.34, pp.21-28, 2004.

T. Nicolai, Colloids and Surfaces B: Biointerfaces, 2015.

M. Boland, H. Singh, and A. Thompson, Milk proteins: from expression to food, 2014.

W. Brown, Light Scattering. Principles and Developments, 1996.
URL : https://hal.archives-ouvertes.fr/hal-01663429

J. S. Higgins and K. C. Benoit, Polymer and Neutron Scattering, 1994.

B. Berne and R. Pecora, Dynamic light scattering With Applications to Chemistry, Biology, and physics, 2000.

W. Brown, Dynamic Light Scattering: The method and some applications, 1993.

V. Sharma, A. Jaishankar, Y. Wang, and G. H. Mckinley, Soft Matter, vol.7, pp.5150-5160, 2011.

M. Pouzot, T. Nicolai, R. W. Visschers, and M. Weijers, Food Hydrocolloids, vol.19, pp.231-238, 2005.

N. F. Carnahan and K. E. Starling, Journal of Chemical Physics, vol.51, pp.635-636, 1969.

T. Phan-xuan, D. Durand, T. Nicolai, L. Donato, C. Schmitt et al., Food Hydrocolloids, vol.34, pp.227-235, 2014.

C. Schmitt, C. Moitzi, C. Bovay, M. Rouvet, L. Bovetto et al., Soft Matter, vol.6, pp.4876-4876, 2010.

D. Vlassopoulos and G. Fytas, High Solid Dispersions, vol.236, pp.1-54, 2010.

I. M. Krieger, Adv. Coll. Int. Sci, vol.3, pp.111-136, 1972.

D. Quemada, Rheologica Acta, vol.16, pp.82-94, 1977.

I. B. Rietveld and D. Bedeaux, Journal of colloid and interface science, vol.235, pp.89-92, 2001.

N. Merlet-lacroix, E. Di-cola, and M. Cloitre, Soft Matter, vol.6, pp.984-993, 2010.

S. Amin, G. V. Barnett, J. A. Pathak, C. J. Roberts, and P. S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion in Colloid & Interface Science, vol.19, pp.438-449, 2014.

W. P. Bryan, The isoionic point of amino acids and proteins, Biochemical Education, vol.6, pp.14-15, 1978.

R. K. Cannan, The Acid-Base Titration of Proteins, Chemical Reviews, vol.30, pp.395-412, 1942.

R. K. Cannan, A. Kibrick, and A. Palmer, The amphoteric properties of egg albumin, Annals of the New York Academy of Sciences, vol.41, pp.243-266, 1941.

R. K. Cannan, A. H. Palmer, and A. C. Kibrick, The hydrogen ion dissociation curve of ?-lactoglobulin, Journal of Biological Chemistry, vol.142, pp.803-822, 1942.

N. Chen, M. Zhao, C. Chassenieux, and T. Nicolai, Structure of Self-assembled Native Soy Globulin in Aqueous Solution as a Function of the Concentration and the pH Food Hydrocolloids, vol.56, pp.417-424, 2016.

A. H. Clark, G. M. Kavanagh, and S. B. Ross-murphy, Globular protein gelationtheory and experiment, Food Hydrocolloids, vol.15, pp.383-400, 2001.

J. De-wit, Nutritional and functional characteristics of whey proteins in food products, Journal of dairy science, vol.81, pp.597-608, 1998.

L. Donato, C. Schmitt, L. Bovetto, and M. Rouvet, Mechanism of formation of stable heat-induced ?-lactoglobulin microgels, International Dairy Journal, vol.19, pp.295-306, 2009.

S. Fredriksson, Scanning isoelectric focusing in small density-gradient columns: IV. The use of deuterium oxide for preparing the density gradient and its effects on isoelectric points of proteins, Journal of Chromatography A, vol.108, pp.153-167, 1975.

F. Guyomarc'h, M. Famelart, G. Henry, M. Gulzar, J. Leonil et al., Current ways to modify the structure of whey proteins for specific functionalities-a review, Dairy Science & Technology, pp.1-20, 2014.

G. Kontopidis, C. Holt, and L. Sawyer, Invited review: ?-lactoglobulin: binding properties, structure, and function, Journal of dairy science, vol.87, pp.785-796, 2004.

M. R. Krebs, G. L. Devlin, and A. M. Donald, Protein particulates: another generic form of protein aggregation?, Biophysical journal, vol.92, pp.1336-1342, 2007.

M. Langton and A. Hermannsson, Fine-stranded and particulate gels of b-lactoglobulin and whey protein at varying pH, Food Hydrocolloids, vol.5, pp.523-539, 1992.

H. Li, L. Zhao, X. D. Chen, and R. Mercadé-prieto, Swelling of whey and egg white protein hydrogels with stranded and particulate microstructures, International journal of biological macromolecules, vol.83, pp.152-159, 2016.

L. Longsworth and C. Jacobsen, An Electrophoretic Study of the Binding of Salt Ions by ?-Lactoglobulin and Bovine Serum Albumin, The Journal of Physical Chemistry, vol.53, pp.126-134, 1949.

R. Mezzenga and P. Fischer, The self-assembly, aggregation and phase transitions of food protein systems in one, two and three dimensions, Reports on Progress in Physics, vol.76, p.46601, 2013.

M. Morand, F. Guyomarc'h, S. Pezennec, and M. Famelart, On how ?-casein affects the interactions between the heat-induced whey protein/?-casein complexes and the casein micelles during the acid gelation of skim milk, International Dairy Journal, vol.21, pp.670-678, 2011.
URL : https://hal.archives-ouvertes.fr/hal-01454176

T. Nicolai, M. Britten, and C. Schmitt, ?-Lactoglobulin and WPI aggregates: Formation, structure and applications, vol.25, pp.1945-1962, 2011.

T. Nicolai and D. Durand, Controlled food protein aggregation for new functionality, Current Opinion in Colloid & Interface Science, vol.18, pp.249-256, 2013.

Y. Nozaki, L. G. Bunville, and C. Tanford, Hydrogen Ion Titration Curves of ?Lactoglobulin1, Journal of the American Chemical Society, vol.81, pp.5523-5529, 1959.

T. Phan-xuan, D. Durand, T. Nicolai, L. Donato, C. Schmitt et al., On the Crucial Importance of the pH for the Formation and Self-Stabilization of Protein Microgels and Strands, vol.27, pp.15092-15101, 2011.

T. Phan-xuan, D. Durand, T. Nicolai, L. Donato, C. Schmitt et al., Heat induced formation of beta-lactoglobulin microgels driven by addition of calcium ions, Food Hydrocolloids, vol.34, pp.227-235, 2014.

. Salis, M. Bostr-m, L. Medda, F. Cugia, B. Barse et al., Measurements and theoretical interpretation of points of zero charge/potential of BSA protein, Langmuir, vol.27, pp.11597-11604, 2011.

C. Schmitt, C. Bovay, A. Vuilliomenet, M. Rouvet, L. Bovetto et al., Multiscale characterization of individualized ?-lactoglobulin microgels formed upon heat treatment under narrow pH range conditions, Langmuir, vol.25, pp.7899-7909, 2009.

S. Sørensen, K. Linderstrøm-lang, and E. Lund, The influence of salts upon the ionisation of egg albumin, The Journal of general physiology, vol.8, pp.543-599, 1927.

C. Tanford and M. L. Wagner, Gelation: principles, models and applications to proteins, Hydrogen Ion Equilibria of Lysozyme1, vol.2, pp.29-91, 1954.

T. Nicolai, M. Britten, C. Schmitt, and . Lactoglobulin,

, Formation, structure and applications, Food Hydrocolloids, vol.25, pp.1945-1962, 2011.

S. Amin, G. V. Barnett, J. A. Pathak, C. J. Roberts, and P. S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion in Colloid & Interface Science, vol.19, issue.5, pp.438-449, 2014.

A. Brodkorb, T. Croguennec, S. Bouhallab, and J. J. Kehoe, Heat-Induced Denaturation, Aggregation and Gelation of Whey Proteins, Advanced Dairy Chemistry McSweeney, P. L

H. ;-o'mahony, J. A. Eds, and . Springer, Journal of Physical Chemistry B, pp.8075-8081, 2016.

, Annexe 149

R. Mezzenga and P. Fischer, The self-assembly, aggregation and phase transitions of food protein systems in one, two and three dimensions, Reports on Progress in Physics, vol.76, issue.4, p.46601, 2013.

N. Mahmoudi, S. Mehalebi, T. Nicolai, D. Durand, and A. Riaublanc, Light-Scattering Study of the Structure of Aggregates and Gels Formed by Heat-Denatured Whey Protein Isolate and b-Lactoglobulin at Neutral pH, J. Agric. Food Chem, vol.55, pp.3104-3111, 2007.

S. Mehalebi, T. Nicolai, and D. Durand, Light scattering study of heat-denatured globular protein aggregates, International Journal of Biological Macromolecules, vol.43, pp.129-164, 2008.

W. Inthavong, A. Kharlamova, C. Chassenieux, and T. Nicolai, Structure and flow of dense suspensions of protein fractal aggregates in comparison with microgels, Soft Matter, 2016.

T. Nicolai, Formation and functionality of self-assembled whey protein microgels

, Colloids and Surfaces B: Biointerfaces, vol.137, pp.32-40, 2015.

G. D. Phillies, Self and tracer diffusion of polymers in solution, 2004.

B. Amsden, Modeling solute solutions in aqueous polymer solutions Polymer, vol.43, pp.1623-1630, 2002.

L. Masaro and X. X. Zhu, Physical models of diffusion for polymer solutions, gels and solids, Prog. Polym. Sci, vol.24, pp.731-775, 1999.

D. W. De-kort, J. P. Van-duynhoven, H. Van-as, and F. Mariette, Nanoparticle diffusometry for quantitative assessment of submicron structure in food biopolymer networks, Trends in Food Science & Technology, vol.42, issue.1, pp.13-26, 2015.

G. Balakrishnan, T. Nicolai, and D. Durand, Relation between the gel structure and the mobility of tracers in globular protein gels, Journal of Colloid and Interface Science, vol.388, pp.293-299, 2012.

P. Croguennoc, T. Nicolai, M. E. Kuil, and J. G. Hollander, Self-diffusion of Native Proteins and Dextran in Heat-set Globular Protein Gels, J. Phys. Chem. B, p.5782, 2001.

R. ;. Colsenet, O. Sã-¶derman, and F. Mariette, Effects of ionic strength and denaturation time on polyethyleneglycol self-diffusion in whey protein solutions and gels visualized by nuclear magnetic resonance, Journal of Physical Chemistry B, vol.54, issue.14, pp.8075-8081, 2006.

R. Colsenet, O. Soderman, and F. Mariette, Pulsed Field Gradient NMR Study of, Annexe, vol.150, issue.16

. Poly, Diffusion in Whey Protein Solutions and Gels, Macromolecules, vol.39, pp.1053-1059, 2006.

B. T. Nguyen, T. Nicolai, C. Chassenieux, C. Schmitt, and L. Bovetto, Heat-induced gelation of mixtures of whey protein isolate and sodium caseinate between pH 5.8 and pH 6.6, Food Hydrocolloids, vol.61, pp.433-441, 2016.

J. S. Higgins and K. C. Benoit, Polymer and Neutron Scattering, 1994.

B. Berne and R. Pecora, Dynamic light scattering With Applications to Chemistry, Biology, and physics, 2000.

J. K. Jonasson, N. Loren, P. Olofsson, M. Nyden, and M. Rudemo, A pixel?based likelihood framework for analysis of fluorescence recovery after photobleaching data, Journal of Microscopy, vol.232, pp.260-269, 2008.

D. M. Soumpasis, Theoretical analysis of fluorescence photobleaching recovery experiments, Biophysical journal, vol.41, issue.1, pp.95-97, 1983.