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Evaluation quantitative par IRM et ¹H SRM de l'exposition à des xénobiotiques chez le petit animal dans un contexte clinique et environnemental

Abstract : Magnetic Resonance Imaging and Spectroscopy (MRI and ¹H MRS) are new methods in toxicology. These tools allow the assessment of the impact of xenobiotics at structural and metabolic levels respectively. In this perspective, this thesis presents two studies in animal models: (i) repeated administrations of linear gadolinium-based contrast agent (gadodiamide) in rat leading to a deposition of gadolinium in deep cerebellar nuclei (DCN); (ii) gestational and lactational exposure to bisphenol A (BPA) and its chlorinated derivatives at low dose (20 μg/kg/day) which are endocrine disruptors.The study of repeated injections of gadodiamide showed a hypersignal of DCN on T1-weighed images at 6and 11 weeks post-injections. In an unprecedented way, it was demonstrated that the hypersignal of DCN persisted for 1 year post-injections. A prolonged increase of total creatine concentration in DCN was also measured by the quantitation ERETIC digital method. Nevertheless, this variation was not confirmed by other conventional quantitation methods (jMRUI and LCModel). A comparison of quantitation methods in ¹H MRS (ERETIC digital, jMRUI and LCModel) was performed in order to elucidate the influence of baseline correction in the processing of in vivo ¹H spectra.Early disturbances caused by chlorinated derivatives of BPA were quantified in young male and female mice perinatally exposed: (i) modifications of hepatic lipid composition which precedes any structural change; (ii)microstructural and metabolic alterations in the hippocampus. A spectroscopic diffusion method was optimized, leading to in vivo experiments in order to characterize those abnormalities of intracellular integrity in the brain of male mice.
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https://tel.archives-ouvertes.fr/tel-01898557
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Submitted on : Thursday, October 18, 2018 - 3:30:30 PM
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Dounia El Hamrani. Evaluation quantitative par IRM et ¹H SRM de l'exposition à des xénobiotiques chez le petit animal dans un contexte clinique et environnemental. Sciences agricoles. Université d'Orléans, 2017. Français. ⟨NNT : 2017ORLE2045⟩. ⟨tel-01898557⟩

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