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Détermination sur tissu endométrial néoplasique de profils d'expression de micro-ARNs associés à l'envahissement ganglionnaire dans le cancer de l'endomètre de type endométrioïde de stade précoce

Abstract : Current histological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely predict lymph node metastases leading to potential over or under treatment. Hence, additional highly sensitive and specific molecular prognostic biomarkers correlated with prognostic factors, such as nodal involvement and lymphovascular space involvement (LVSI), are needed to better adapt surgical management and adjuvant therapies. We fstudied by microarray analysis microRNA expression profiles of formalin-fixed paraffin-embedded grade 1–2 supposed early-stage endometrioid adenocarcinomas specimen. The expression levels of 5 microRNAs (miR-34c-5p, -375, -184, -34c-3p, et -34b-5p) were lower in the EC with positive nodal status compared to those with negative nodal status. The expression levels of 3 microRNAs (miR-34c-5p, -23b-5p, et -23c) were lower in the EC with positive LVSI compared to those with negative LVSI. A quantitative reverse transcriptase–PCR assay was used to determine micro-RNAs thresholds correlated with prognostic factors. Women with a microRNA-184-fold change <0.30 were more likely to have positive lymph node (n=6; 60.0%) compared with those with a microRNA-184-fold change >0.30 (n=3; 11.5%), p=0.006. Women with a microRNA-34c-5p fold change <0.15 were more likely to have positive LVSI status (n=12; 92.3%) compared with those with a microRNA-34c-5p fold change >0.15 (n=0; 0.0%), p<0.001. These microRNA expression profiles may provide a basis for further studies of the micro-RNA function in endometrioid adenocarcinoma, and be used as a diagnostic tool.
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https://tel.archives-ouvertes.fr/tel-01895258
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Submitted on : Monday, October 15, 2018 - 1:02:02 AM
Last modification on : Sunday, October 25, 2020 - 2:51:05 PM
Long-term archiving on: : Wednesday, January 16, 2019 - 1:01:08 PM

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2017PA066198.pdf
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  • HAL Id : tel-01895258, version 1

Citation

Geoffroy Canlorbe. Détermination sur tissu endométrial néoplasique de profils d'expression de micro-ARNs associés à l'envahissement ganglionnaire dans le cancer de l'endomètre de type endométrioïde de stade précoce. Physiologie [q-bio.TO]. Université Pierre et Marie Curie - Paris VI, 2017. Français. ⟨NNT : 2017PA066198⟩. ⟨tel-01895258⟩

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