Skip to Main content Skip to Navigation
Theses

Effets des rayonnements ionisants sur des biomolécules en solution : vers une caractérisation des dommages à l'échelle moléculaire

Abstract : In this thesis, we have developed and characterized a new type of gas phase source of biomolecules which based on non-resonant laser desorption on microdroplets directly under vacuum. This device will eventually allow us to open an original way to study the effects of ionizing radiation on organic molecules from a physical point of view. We will present in detail this device with which we transfered with sucess under vacuum,in a non-destructively way, biomolecules and non-covalent complexes in a mass range of the order of kDa and we assigned them with time-of-ight mass spectrometry. We will show in particular the technical challenges that we had to overcome in order to allow the transfer of microdroplets under vacuum and how by simulation of the spectrometer, we have been able to highly optimize the collection parameters of the desorbed molecular species and the mass resolution of our system, by replacing, in the desorption zone,delayed extraction by a quadrupole electrostatic trap. Finally, we will describe the way in which this device, coupled to a simply charged ion irradiation platform with an energy of the order of the keV, will enable us to characterizeon a molecular scale the damage due to radio-induced radical chemistry mechanisms.
Complete list of metadatas

Cited literature [116 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-01891580
Contributor : Abes Star :  Contact
Submitted on : Wednesday, October 10, 2018 - 9:28:06 AM
Last modification on : Saturday, February 15, 2020 - 2:04:03 AM

File

edgalilee_th_2017_leite.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-01891580, version 2

Collections

Citation

Serge Leite. Effets des rayonnements ionisants sur des biomolécules en solution : vers une caractérisation des dommages à l'échelle moléculaire. Chimie-Physique [physics.chem-ph]. Université Sorbonne Paris Cité, 2017. Français. ⟨NNT : 2017USPCD025⟩. ⟨tel-01891580v2⟩

Share

Metrics

Record views

263

Files downloads

834