, Dans la troisième fraction analysée, nous avons montré six pics (Figure 37), avec des masses [M+H] + de 413,2456 Da Da Da, p.851600526224
, , 1322.
6224 Da a été identifiée grâce à la présence de pics mono, di et tri-chargés et des adduits sodium et potassium correspondants. La molécule de masse 1322,6680 Da a été identifiée grâce à la présence de pics mono et di-chargés et des adduits sodium et potassium correspondants, p.52, 1221. ,
apparait pas sur le spectre, les adduits sodium et potassium correspondant sont également visibles. L'analyse du spectre a également montré la présence de massif isotopique car une différence de masse (+ 1 Da, + 0.5 Da ou + 0,33 Da) était visible, due à la présence de 13 C. La présence d'adduits sodium [M + Na] + sur chacune des molécules est également visible avec un écart de masse de 22 Da ou de 11 Da pour les molécules di-chargées et 7,3 Da pour la molécule tri-chargée. La présence d'adduits potassium ,
, sur le spectre sont répertoriées dans le tableau 8 Ces résultats confirment la présence d'une molécule tri-chargée, d'une molécule di-chargée et d'une molécule mono-chargée dans l'échantillon dans les conditions de l'expérimentation. 2.2.1.1. Co-culture S. aureus -A. castellanii La co-culture de S. aureus en présence d'A. castellanii induit un effet positif sur la croissance bactérienne, Les différentes masses expérimentales
, Numération des UFC de S. aureus en co-culture avec A, Figure, vol.46
, Toutes les valeurs correspondent à la moyenne de neuf répétitions. Les valeurs avec les étoiles sont significativement différentes
, Co-culture S. aureus -V. vermiformis La co-culture de S. aureus avec V. vermiformis montre également un effet positif sur la croissance bactérienne, plus précoce que lors de la co-culture avec A. castellanii. En effet, une augmentation significative de la croissance de S. aureus est observée, à partir de 3 h de coculture
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