Skip to Main content Skip to Navigation

Interactions amibes libres / micro-organismes : préférence trophique et étude comparative avec les macrophages

Abstract : Free living amoebae (FLA) are protoza found in various environments where they can feed by phagocytosis on bacteria, fungi, viruses or other protozoa. Acanthamoebae castellanii was used as the main model in this thesis, divided in two parts. The first part of the thesis relied on the trophic preference of amoebae, in presence of different microorganisms. Amongst them, two bacteria, Klebsiella pneumoniae and Staphylococcus aureus, appeared as the most attractive for the studied protozoa. Bacterial extracts have been fractionned and their study has shown the protein nature of the chemoattractants involved in this interspecies crosstalk.Literature data have reported similarities between A. castellanii and other phagocytic cells such as macrophages. The second part of the thesis allowed us to compare phagocytic activities of A. castellanii and Thp-1 macrophagic cells towards four microorganisms: the filamentous fungus Aspergillus fumigatus, the bacteria Klebsiella pneumoniae and Staphylococcus aureus and an Adenovirus B3 serotype. The influence of the two phagocytic cells on the microorganisms' growth has also been investigated. This has evidenced the behavior differences between FLA and macrophages towards pathogenic microorganisms, showing that results obtained by studying amoebae and microorganisms relationships could not be extrapolated in all cases to the relationships between macrophages and microorganisms.
Complete list of metadata

Cited literature [315 references]  Display  Hide  Download
Contributor : Abes Star :  Contact
Submitted on : Monday, August 20, 2018 - 3:01:07 PM
Last modification on : Friday, January 29, 2021 - 1:39:09 PM
Long-term archiving on: : Wednesday, November 21, 2018 - 1:20:12 PM


Version validated by the jury (STAR)


  • HAL Id : tel-01858349, version 1



Elodie Maisonneuve. Interactions amibes libres / micro-organismes : préférence trophique et étude comparative avec les macrophages. Ecologie, Environnement. Université de Poitiers, 2017. Français. ⟨NNT : 2017POIT2257⟩. ⟨tel-01858349⟩



Record views


Files downloads