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, utile pour une utilisation in vivo de ces nanoparticules si l'on veut éviter, ou du moins retarder, la capture par le système réticulo?endothélial

, Le diamètre hydrodynamique mesuré en fonction du pH montre l'intérêt de recouvrir les particules par du PO?PEG : celles?ci restent stables avec un diamètre hydrodynamique proche de 15 nm à tout pH, contrairement aux USNPLP nues (Figure 6.6.b) On observe le même effet de passivation et d'augmentation de la stabilité par recouvrement avec du PO?PEG en mesurant le potentiel?? en fonction du pH (Figure 6.6.c) : il reste

. De, diamètre hydrodynamique stable autour de 16 nm dans des conditions physiologiques (Figure 6.6.e) pendant plusieurs dizaine d'heures (Figure 6.6.f) ce qui est un argument rassurant concernant leur utilisation dans des milieux biologiques. iii, Propriétés de luminescence et utilisation in vivo Les propriétés de luminescence des USNPLP ont tout d'abord été déterminées in vitro

, a) Spectres d'émission d'USNPLP non dopées (courbe ?) et dopées avec du chrome (III) (courbe ?). b) Profil d'absorbance des USNPLP recouvertes de PO?PEG en solution. c?d) Déclins de luminescence des USNPLP sous forme de poudre (c) ou en solution

L. , ZGO non dopés sont connus pour émettre dans le bleu (courbe ?, Figure 6, p.449

3. Tandis-que-le-dopage-avec-du-cr, obtenir une émission dans la première fenêtre de transparence des tissus, autout de 700 nm (courbe ?, Figure 6.7.a) Ils absorbent fortement dans les UV

, Aire moyenne des boucles (pixels)

, Annexe III : optimisation de la synthèse hydrothermale Graphique 8.11 Evolution de la distribution du diamètre hydrodynamique des ZGO avant (courge rouge) et après (courge verte)

, Distribution du diamètre hydrodynamique des ZGO@SiO2 après un traitement de 24 h dans NaOH 5 mM à température ambiante (courge rouge) ou de 12 h dans KOH 1 M à 12°C après (courge verte), Graphique 8.12

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